Construct: ORF ccsbBroad304_15866
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012607.1_s304c1, BRDN0000404886
- Derived from:
- ccsbBroadEn_15866
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ING3 (54556)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15866
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54556 | ING3 | inhibitor of growth family ... | XM_017012369.2 | 97.1% | 95.6% | 268C>G;269_270insT;273_273delCinsTGGAAT |
| 2 | human | 54556 | ING3 | inhibitor of growth family ... | NM_198267.2 | 95% | 95.6% | 268C>G;270_274delGCACT;276_277insTTTGGAAT |
| 3 | human | 54556 | ING3 | inhibitor of growth family ... | NM_019071.3 | 22% | 21.2% | (many diffs) |
| 4 | human | 54556 | ING3 | inhibitor of growth family ... | XR_001744819.1 | 13.3% | 1_112del;392_2085del | |
| 5 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_001311061.1 | 20.9% | 21.7% | (many diffs) |
| 6 | mouse | 71777 | Ing3 | inhibitor of growth family,... | NM_023626.4 | 20.4% | 21.1% | (many diffs) |
| 7 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_006505166.3 | 15.1% | 15.4% | (many diffs) |
| 8 | mouse | 71777 | Ing3 | inhibitor of growth family,... | XM_017321747.1 | 12.6% | 12.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 345
- ORF length:
- 279
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtacctagaa gactatctgg aaatgattga gcagcttcct atggatctgc 121 gggaccgctt cacggaaatg cgcgagatgg acctgcaggt gcagaatgca atggatcaac 181 tagaacaaag agtcagtgaa ttctttatga atgcaaagaa aaataaacct gagtggaggg 241 aagagcaaat ggcatccatc aaaaaagact actataaagc tttggaagat gcagatgaga 301 aggttcagtt ggcaaaccag atatatgact tggatctttg gaattnncca actttcttgt 361 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 421 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a