Construct: ORF ccsbBroad304_15897
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004207.1_s304c1, BRDN0000409870
- Derived from:
- ccsbBroadEn_15897
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJA4 (55466)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15897
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_001130183.1 | 64.5% | 64.3% | 1_393del;596A>G |
| 2 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_001130182.1 | 60.1% | 59.9% | 1_474del;677A>G |
| 3 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | XM_005254535.3 | 60.1% | 59.9% | 1_474del;677A>G |
| 4 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | XM_006720601.1 | 60.1% | 59.9% | 1_474del;677A>G |
| 5 | human | 55466 | DNAJA4 | DnaJ heat shock protein fam... | NM_018602.3 | 56% | 55.8% | 1_561del;764A>G |
| 6 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | NM_021422.4 | 53% | 54.1% | (many diffs) |
| 7 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | XM_006511325.3 | 37.9% | 38.7% | (many diffs) |
| 8 | mouse | 58233 | Dnaja4 | DnaJ heat shock protein fam... | XR_001778951.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca gatccacatc cagcagatcg ggccgggcat ggtacagcag atccagaccg 121 tgtgcatcga gtgcaagggc cagggtgagc gcatcaaccc caaggaccgc tgcgagagct 181 gcagcggggc caaggtgatc cgtgagaaga agattatcga ggtacatgtt gaaaaaggta 241 tgaaagatgg gcaaaagata ctatttcgtg gagaaggaga tcaggagcct gagctggagc 301 ctggtgatgt cataattgtg cttgatcaga aggatcatag tgtctttcag agacgaggcc 361 atgacttgat catgaaaatg aaaattcagc tttctgaagc tctttgtggc ttcaagaaga 421 cgataaaaac attggacaat cgaattcttg ttattacatc caaagcaggt gaggtgataa 481 agcacgggga cctgagatgc gtgcgcgatg aaggaatgcc catctacaaa gcacccctgg 541 aaaaagggat tctgatcata cagtttttag taatctttcc tgaaaaacac tggctttctc 601 tggaaaagct tcctcagctg gaagctttac tccctcctcg acagaaagtg aggattacag 661 atgacatgga tcaggtggag ctgaaggagt tttgtcccaa tgagcagaac tggcgtcagc 721 acagggaggc ctacgaggag gacgaagacg ggccccaggc tggagtgcag tgccagacgg 781 catnnccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 841 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 901 tctggatta