Construct: ORF ccsbBroad304_16130

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001693.1_s304c1, BRDN0000399590
Derived from:
ccsbBroadEn_16130
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SKA3 (221150)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16130
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221150 SKA3 spindle and kinetochore ass... XM_005266288.4 99.7% 99.6% 514A>G;840G>A
2 human 221150 SKA3 spindle and kinetochore ass... XM_011534994.2 88.5% 88.4% 1_126del;640A>G;966G>A
3 human 221150 SKA3 spindle and kinetochore ass... NM_145061.6 79.9% 79.8% 1_246del;760A>G;1086G>A
4 human 221150 SKA3 spindle and kinetochore ass... NM_001166017.2 73% 69.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1056
ORF length:
990
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga aaaaaattca atggatatta tgaaaataag agagtatttc cagaagtatg
121gatatagtcc acgtgtcaag aaaaattcag tacacgagca agaagccatt aactctgacc
181cagagttgtc taattgtgaa aattttcaga agactgatgt gaaagatgat ctgtctgatc
241ctcctgttgc aagcagttgt atttctgaga agtctccacg tagtccacaa ctttcagatt
301ttggacttga gcggtacatc gtatcccaag ttctaccaaa ccctccacag gcagtgaaca
361actataagga agagcccgta attgtaaccc cacctaccaa acaatcacta gtaaaagtac
421taaaaactcc aaaatgtgca ctaaaaatgg atgattttga gtgtgtaact cctaaattag
481aacactttgg tatctctgaa tatactatgt gtttaaatga agattacaca atgggactta
541aaaatgcgag gaataataaa agtgaggagg ccatagatgc agaatccagg ctcaatgata
601atgtttttgc cactcccagc cccatcatcc agcagttgga aaaaagtgat gccgaatata
661ccaactctcc tttggtacct acattctgta ctcctggttt gaaaattcca tctacaaaga
721acagcatagc tttggtatcc acaaattacc cattatcaaa aacaaatagt tcatcaaatg
781atttggaagt tgaagatcgt acttcgttgg ttttaaattc agacacatgc tttgagaatt
841taacagatcc ctcttcacct acgatttctt cttatgagaa tctgctcaga acacctacac
901ctccagaagt aactaaaatt ccagaagata ttctccagct tttatcaaaa tacaactcaa
961acctagctac tccaatagca attaaagcag tgccacccag taaaaggttc cttaaacatg
1021gacagaacat ccgagatgtc agcaacaaag aaaactnncc aactttcttg tacaaagtgg
1081ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1141ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)

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