Construct: ORF ccsbBroad304_16153

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011544.1_s304c1, BRDN0000401481
Derived from:
ccsbBroadEn_16153
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRIM42 (287015)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_16153
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 287015 TRIM42 tripartite motif containing 42 NM_152616.5 99.8% 99.5% 731A>G;1423G>A;1736C>A
2 human 287015 TRIM42 tripartite motif containing 42 XM_011512740.3 96.8% 95.3% (many diffs)
3 human 287015 TRIM42 tripartite motif containing 42 XR_001740121.2 89.2% (many diffs)
4 human 287015 TRIM42 tripartite motif containing 42 XR_001740122.2 87.4% (many diffs)
5 mouse 78911 Trim42 tripartite motif-containing 42 NM_030219.2 85.6% 87.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2238
ORF length:
2169
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaaactgct atgtgcgttt gctgtccatg ttgtacatgg cagagatgtt
121gtcctcagtt atgctcctgt ctgtgctgca agttcatctt cacctcagag cggaactgca
181cctgcttccc ctgcccttac aaagatgagc ggaactgcca gttctgccac tgcacctgtt
241ctgagagccc caactgccat tggtgttgct gctcttgggc caatgatccc aactgtaagt
301gctgctgcac agccagcagc aatctcaact gctactacta tgagagccgc tgctgccgca
361ataccatcat cactttccac aagggccgcc tcaggagcat ccatacctcc tccaagactg
421ccctgcgcac tgggagcagc gatacccagg tggatgaagt aaagtcaata ccagccaaca
481gtcacctggt gaaccacctc aattgcccca tgtgcagccg gctgcgcctg cactcattca
541tgctgccctg caaccacagc ctgtgcgaga agtgcctgcg gcagctgcag aagcacgccg
601aggtcaccga gaacttcttc atcctcatct gcccagtgtg cgaccgctcg cactgcatgc
661cctacagcaa caagatgcag ctgcccgaga actacctgca cgggcgtctc accaagcgct
721acatgcagga gcacggctac ctcaagtggc gctttgaccg ctcctccggg cccatcctct
781gccaggtctg ccgcaacagg cgcatcgctt acaagcgctg catcacctgc cgcctcaacc
841tgtgcaacga ctgcctcaag gccttccact cggatgtggc catgcaagac cacgtctttg
901tggacaccag cgccgaggaa caggacgaga agatctgcat ccaccaccca tccagccgca
961tcatcgagta ctgccgcaat gacaacaaat tgctctgcac cttctgcaag ttctctttcc
1021acaatggcca cgacaccatt agcctcatcg acgcctgctc cgagagggcc gcctcactct
1081tcagcgccat cgccaagttc aaagcagtcc gatatgaaat tgataatgac ctaatggaat
1141tcaacatctt aaaaaacagc tttaaagctg acaaggaggc aaagcgaaaa gagatcagaa
1201atggctttct caagttgcgc agcattcttc aggagaaaga gaagatcatc atggagcaga
1261tagagaatct agaagtgtcc aggcagaagg aaattgaaaa atatgtgtat gttacaacca
1321tgaaagtgaa cgagatggat ggtctgatcg cctactccaa ggaagccctg aaggagactg
1381gccaggtggc attcctgcag tcagccaaga tcctggtgga ccagatcgag gacggcatcc
1441agaccaccta caggcctgac ccacagctcc ggctgcactc aataaactac atgcccttgg
1501actttgttga gctttccagt gccatccatg agctcttccc cacagggccc aagaaggtac
1561gctcctcagg ggactccctg ccctccccct accccgtgca ctcagaaaca atgattgcca
1621ggaaggtcac tttcagcacc cacagcctcg gcaaccagca catataccag cgaagctcct
1681ccatgttgtc cttcagcaac actgacaaga aggccaaggt gggtctggag gcctgtggga
1741gagcccagtc agccaccccc gccaaaccca cagacggcct ctacacctac tggagtgctg
1801gagaagacag ccagtctgta cagaacagca gcagcttcca caactggtac tcattcaacg
1861atggctctgt gaagacccca ggcccaattg ttatctacca gactctggtg tacccaagag
1921ctgccaaggt ttactggaca tgtccagcag aagacgtgga ctcttttgag atggaattct
1981atgaagtcat tacttctcct cctaacaacg tacaaatgga gctctgtgga caaattcggg
2041acataatgca gcaaaatctg gagctgcaca acctgacccc caacacagaa tacgtgttta
2101aagttagagc catcaatgat aatggtcctg ggcaatggag tgatatctgc aaggtggtaa
2161caccagatgg acatgggaag aaccgagcta agtggggcct gctgaagaat atccagtctg
2221ccctccagaa gcacttctnn ccaactttct tgtacaaagt ggttggtaag cctatcccta
2281accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2341agtcgacaat caacctctgg atta

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