Construct: ORF ccsbBroad304_99993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORFC00003.3_s304c1, BRDN0000412230
- Derived from:
- BRDN0000464768
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- Luciferase (Luciferase)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_99993
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | Luciferase | Luciferase | Hahn Lab Luciferase | Luciferase.1 | 76.8% | 56.5% | (many diffs) |
| 2 | CONTROL | LUCIFERASE | LUCIFERASE | promegaLuc | promegaLuc.1 | 76.8% | 56.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1047
- ORF length:
- 978
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg 121 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg 181 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg 241 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa 301 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta 361 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta 421 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga 481 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt 541 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg 601 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact 661 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga 721 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt 781 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat 841 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc 901 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa 961 gcactctgat tgacaaatac gatttatcta GTTTCCCCAC AACGGAGTGC GTGGCGTCTG 1021 CATTTAGTCA AGCTTGTCTC TTGATGTAAT TCCCCTTGGA AGTTCCCCCT ATAGAGTTGA 1081 ATCTTGGGTC ATTCTCAAGC ATCATACACT GGTTATAAGC TTGGTAATCC CATTTCAGGT 1141 GTCGTGAGAA TAGCATCGAT TGATCAACAT GTTTGTTCAT AAAAGTTGGC ATGGCCCCAA 1201 GCAAGAGGAG CCACCAAGGC TTCGGCTTCG TCTTCTGCTT CATGCACAGT GATATCACGG 1261 ACATCAACCT CCGGGACAAC CTCCGTGAGC AGTTCATGGA GTTCTCCAGC CCCATCTCGA 1321 ACGCAGAGGA GCTCAACATC ATGCTAGGCT GAGTCGGTGG TGGCTCCCGN TGAATTGGAN 1381 NNCATTGACA AAGGCCGAGA GGCTNTTGNT NNNACNNCCC AACATCTTCN ANGNAGNNNT 1441 NGCAGNTNTN CNNNANNANG ANGNNGGTGN NCTNNCNGNC NCCTCTGGCC ACATCTCAGG 1501 GTTNNTGGCA TGGGGTGGCT NTTGGNGCNN GGAAAGACGA TGNCGGNNNA AGNNNTCAGT 1561 CTGATGTNNA TTACNTCGCC AGNCNNGTNN CAACCGCGAA AAAGTTGCGC GGAGGAGTNG 1621 TGTTNGNGGA CGAAGTACCG AAAGGTCTTA CCGGAAAACT CGACGCAANA ANAATCAGAG 1681 AGATCCTCAT AAAGGCCAAG AAGGGCGGAA AGATCGCCGN NTNNCCAACT TTCTTGTACA 1741 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1801 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1861 AGGACGATCA CCATTCCCGT TTTTTCAACT AACGCGTTAA GTCGACAATC AACCTCTGGA 1921 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 1981 TGGATACGCT GCTTTAATGC CTTGTATCAT GCTAGCCCCT GGT