Construct: ORF ccsbBroad304_99993

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORFC00003.3_s304c1, BRDN0000412230
Derived from:
BRDN0000464768
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
Luciferase (Luciferase)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_99993
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 CONTROL Luciferase Luciferase Hahn Lab Luciferase Luciferase.1 76.8% 56.5% (many diffs)
2 CONTROL LUCIFERASE LUCIFERASE promegaLuc promegaLuc.1 76.8% 56.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1047
ORF length:
978
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
121aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
181gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
241aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
301tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
361tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
421tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
481acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
541accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
601aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
661cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
721gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
781taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
841gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
901aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
961gcactctgat tgacaaatac gatttatcta GTTTCCCCAC AACGGAGTGC GTGGCGTCTG
1021CATTTAGTCA AGCTTGTCTC TTGATGTAAT TCCCCTTGGA AGTTCCCCCT ATAGAGTTGA
1081ATCTTGGGTC ATTCTCAAGC ATCATACACT GGTTATAAGC TTGGTAATCC CATTTCAGGT
1141GTCGTGAGAA TAGCATCGAT TGATCAACAT GTTTGTTCAT AAAAGTTGGC ATGGCCCCAA
1201GCAAGAGGAG CCACCAAGGC TTCGGCTTCG TCTTCTGCTT CATGCACAGT GATATCACGG
1261ACATCAACCT CCGGGACAAC CTCCGTGAGC AGTTCATGGA GTTCTCCAGC CCCATCTCGA
1321ACGCAGAGGA GCTCAACATC ATGCTAGGCT GAGTCGGTGG TGGCTCCCGN TGAATTGGAN
1381NNCATTGACA AAGGCCGAGA GGCTNTTGNT NNNACNNCCC AACATCTTCN ANGNAGNNNT
1441NGCAGNTNTN CNNNANNANG ANGNNGGTGN NCTNNCNGNC NCCTCTGGCC ACATCTCAGG
1501GTTNNTGGCA TGGGGTGGCT NTTGGNGCNN GGAAAGACGA TGNCGGNNNA AGNNNTCAGT
1561CTGATGTNNA TTACNTCGCC AGNCNNGTNN CAACCGCGAA AAAGTTGCGC GGAGGAGTNG
1621TGTTNGNGGA CGAAGTACCG AAAGGTCTTA CCGGAAAACT CGACGCAANA ANAATCAGAG
1681AGATCCTCAT AAAGGCCAAG AAGGGCGGAA AGATCGCCGN NTNNCCAACT TTCTTGTACA
1741AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1801AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1861AGGACGATCA CCATTCCCGT TTTTTCAACT AACGCGTTAA GTCGACAATC AACCTCTGGA
1921TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG
1981TGGATACGCT GCTTTAATGC CTTGTATCAT GCTAGCCCCT GGT

Download FASTA (ORF) (Full)