Construct: ORF ccsbBroadEn_00223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013609.1_s300c1, BRDN0000389556
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CASP9 (842)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 842 | CASP9 | caspase 9 | NM_001229.5 | 100% | 100% | |
| 2 | human | 842 | CASP9 | caspase 9 | XM_011542273.3 | 93.3% | 90.7% | (many diffs) |
| 3 | human | 842 | CASP9 | caspase 9 | NM_032996.3 | 80% | 80% | 0_1ins249 |
| 4 | human | 842 | CASP9 | caspase 9 | XM_005246014.2 | 80% | 80% | 0_1ins249 |
| 5 | human | 842 | CASP9 | caspase 9 | NM_001278054.1 | 63.9% | 63.9% | 417_418ins450 |
| 6 | human | 842 | CASP9 | caspase 9 | NR_102732.1 | 40.3% | 1_245del;698_755del;1552_3096del | |
| 7 | human | 842 | CASP9 | caspase 9 | NR_102733.1 | 36.4% | 1_245del;522_523ins140;1354_2898del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1314
- ORF length:
- 1248
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA CGAAGCGGAT CGGCGGCTCC TGCGGCGGTG CCGGCTGCGG CTGGTGGAAG 121 AGCTGCAGGT GGACCAGCTC TGGGACGCCC TGCTGAGCCG CGAGCTGTTC AGGCCCCATA 181 TGATCGAGGA CATCCAGCGG GCAGGCTCTG GATCTCGGCG GGATCAGGCC AGGCAGCTGA 241 TCATAGATCT GGAGACTCGA GGGAGTCAGG CTCTTCCTTT GTTCATCTCC TGCTTAGAGG 301 ACACAGGCCA GGACATGCTG GCTTCGTTTC TGCGAACTAA CAGGCAAGCA GCAAAGTTGT 361 CGAAGCCAAC CCTAGAAAAC CTTACCCCAG TGGTGCTCAG ACCAGAGATT CGCAAACCAG 421 AGGTTCTCAG ACCGGAAACA CCCAGACCAG TGGACATTGG TTCTGGAGGA TTTGGTGATG 481 TCGGTGCTCT TGAGAGTTTG AGGGGAAATG CAGATTTGGC TTACATCCTG AGCATGGAGC 541 CCTGTGGCCA CTGCCTCATT ATCAACAATG TGAACTTCTG CCGTGAGTCC GGGCTCCGCA 601 CCCGCACTGG CTCCAACATC GACTGTGAGA AGTTGCGGCG TCGCTTCTCC TCGCTGCATT 661 TCATGGTGGA GGTGAAGGGC GACCTGACTG CCAAGAAAAT GGTGCTGGCT TTGCTGGAGC 721 TGGCGCAGCA GGACCACGGT GCTCTGGACT GCTGCGTGGT GGTCATTCTC TCTCACGGCT 781 GTCAGGCCAG CCACCTGCAG TTCCCAGGGG CTGTCTACGG CACAGATGGA TGCCCTGTGT 841 CGGTCGAGAA GATTGTGAAC ATCTTCAATG GGACCAGCTG CCCCAGCCTG GGAGGGAAGC 901 CCAAGCTCTT TTTCATCCAG GCCTGTGGTG GGGAGCAGAA AGACCATGGG TTTGAGGTGG 961 CCTCCACTTC CCCTGAAGAC GAGTCCCCTG GCAGTAACCC CGAGCCAGAT GCCACCCCGT 1021 TCCAGGAAGG TTTGAGGACC TTCGACCAGC TGGACGCCAT ATCTAGTTTG CCCACACCCA 1081 GTGACATCTT TGTGTCCTAC TCTACTTTCC CAGGTTTTGT TTCCTGGAGG GACCCCAAGA 1141 GTGGCTCCTG GTACGTTGAG ACCCTGGACG ACATCTTTGA GCAGTGGGCT CACTCTGAAG 1201 ACCTGCAGTC CCTCCTGCTT AGGGTCGCTA ATGCTGTTTC GGTGAAAGGG ATTTATAAAC 1261 AGATGCCTGG TTGCTTTAAT TTCCTCCGGA AAAAACTTTT CTTTAAAACA TCATACCCAA 1321 CTTTCTTGTA CAAAGTtggc attataagaa agcattgctt atcaatttgt tgcaacgaac