Construct: ORF ccsbBroadEn_00282

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009291.1_s300c1, BRDN0000391978
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CDKN1A (1026)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1026 CDKN1A cyclin dependent kinase inh... NM_000389.5 100% 100%
2 human 1026 CDKN1A cyclin dependent kinase inh... NM_001220777.1 100% 100%
3 human 1026 CDKN1A cyclin dependent kinase inh... NM_001220778.1 100% 100%
4 human 1026 CDKN1A cyclin dependent kinase inh... NM_078467.2 100% 100%
5 human 1026 CDKN1A cyclin dependent kinase inh... NM_001291549.2 82.8% 82.8% 1_102del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
558
ORF length:
492
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGTC AGAACCGGCT GGGGATGTCC GTCAGAACCC ATGCGGCAGC AAGGCCTGCC
121GCCGCCTCTT CGGCCCAGTG GACAGCGAGC AGCTGAGCCG CGACTGTGAT GCGCTAATGG
181CGGGCTGCAT CCAGGAGGCC CGTGAGCGAT GGAACTTCGA CTTTGTCACC GAGACACCAC
241TGGAGGGTGA CTTCGCCTGG GAGCGTGTGC GGGGCCTTGG CCTGCCCAAG CTCTACCTTC
301CCACGGGGCC CCGGCGAGGC CGGGATGAGT TGGGAGGAGG CAGGCGGCCT GGCACCTCAC
361CTGCTCTGCT GCAGGGGACA GCAGAGGAAG ACCATGTGGA CCTGTCACTG TCTTGTACCC
421TTGTGCCTCG CTCAGGGGAG CAGGCTGAAG GGTCCCCAGG TGGACCTGGA GACTCTCAGG
481GTCGAAAACG GCGGCAGACC AGCATGACAG ATTTCTACCA CTCCAAACGC CGGCTGATCT
541TCTCCAAGAG GAAGCCCTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
601gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)