Construct: ORF ccsbBroadEn_00481
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005827.1_s300c1, BRDN0000389278
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EEF1A2 (1917)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1917 | EEF1A2 | eukaryotic translation elon... | NM_001958.5 | 100% | 100% | |
| 2 | human | 1915 | EEF1A1 | eukaryotic translation elon... | NM_001402.6 | 77.8% | 92.2% | (many diffs) |
| 3 | mouse | 13628 | Eef1a2 | eukaryotic translation elon... | NM_007906.2 | 89.1% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1455
- ORF length:
- 1389
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG CAAGGAGAAG ACCCACATCA ACATCGTGGT CATCGGCCAC GTGGACTCCG 121 GAAAGTCCAC CACCACGGGC CACCTCATCT ACAAATGCGG AGGTATTGAC AAAAGGACCA 181 TTGAGAAGTT CGAGAAGGAG GCGGCTGAGA TGGGGAAGGG ATCCTTCAAG TATGCCTGGG 241 TGCTGGACAA GCTGAAGGCG GAGCGTGAGC GCGGCATCAC CATCGACATC TCCCTCTGGA 301 AGTTCGAGAC CACCAAGTAC TACATCACCA TCATCGATGC CCCCGGCCAC CGCGACTTCA 361 TCAAGAACAT GATCACGGGT ACATCCCAGG CGGACTGCGC AGTGCTGATC GTGGCGGCGG 421 GCGTGGGCGA GTTCGAGGCG GGCATCTCCA AGAATGGGCA GACGCGGGAG CATGCCCTGC 481 TGGCCTACAC GCTGGGTGTG AAGCAGCTCA TCGTGGGCGT GAACAAAATG GACTCCACAG 541 AGCCGGCCTA CAGCGAGAAG CGCTACGACG AGATCGTCAA GGAAGTCAGC GCCTACATCA 601 AGAAGATCGG CTACAACCCG GCCACCGTGC CCTTTGTGCC CATCTCCGGC TGGCACGGTG 661 ACAACATGCT GGAGCCCTCC CCCAACATGC CGTGGTTCAA GGGCTGGAAG GTGGAGCGTA 721 AGGAGGGCAA CGCAAGCGGC GTGTCCCTGC TGGAGGCCCT GGACACCATC CTGCCCCCCA 781 CGCGCCCCAC GGACAAGCCC CTGCGCCTGC CGCTGCAGGA CGTGTACAAG ATTGGCGGCA 841 TTGGCACGGT GCCCGTGGGC CGGGTGGAGA CCGGCATCCT GCGGCCGGGC ATGGTGGTGA 901 CCTTTGCGCC AGTGAACATC ACCACTGAGG TGAAGTCAGT GGAGATGCAC CACGAGGCTC 961 TGAGCGAAGC TCTGCCCGGC GACAACGTCG GCTTCAATGT GAAGAACGTG TCGGTGAAGG 1021 ACATCCGGCG GGGCAACGTG TGTGGGGACA GCAAGTCTGA CCCGCCGCAG GAGGCTGCTC 1081 AGTTCACCTC CCAGGTCATC ATCCTGAACC ACCCGGGGCA GATTAGCGCC GGCTACTCCC 1141 CGGTCATCGA CTGCCACACA GCCCACATCG CCTGCAAGTT TGCGGAGCTG AAGGAGAAGA 1201 TTGACCGGCG CTCTGGCAAG AAGCTGGAGG ACAACCCCAA GTCCCTGAAG TCTGGAGACG 1261 CGGCCATCGT GGAGATGGTG CCGGGAAAGC CCATGTGTGT GGAGAGCTTC TCCCAGTACC 1321 CGCCTCTCGG CCGCTTCGCC GTGCGCGACA TGAGGCAGAC GGTGGCCGTA GGCGTCATCA 1381 AGAACGTGGA GAAGAAGAGC GGCGGCGCCG GCAAGGTCAC CAAGTCGGCG CAGAAGGCGC 1441 AGAAGGCGGG CAAGTGCCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 1501 tatcaatttg ttgcaacgaa c