Construct: ORF ccsbBroadEn_00508
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010787.1_s300c1, BRDN0000457301
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB3 (2049)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2049 | EPHB3 | EPH receptor B3 | NM_004443.4 | 100% | 100% | |
| 2 | mouse | 13845 | Ephb3 | Eph receptor B3 | NM_010143.1 | 88.2% | 96.1% | (many diffs) |
| 3 | mouse | 13845 | Ephb3 | Eph receptor B3 | XM_006521788.1 | 83.6% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3060
- ORF length:
- 2994
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC CAGAGCCCGC CCGCCGCCGC CGCCGTCGCC GCCGCCGGGG CTTCTGCCGC 121 TGCTCCCTCC GCTGCTGCTG CTGCCGCTGC TGCTGCTGCC CGCCGGCTGC CGGGCGCTGG 181 AAGAGACCCT CATGGACACA AAATGGGTAA CATCTGAGTT GGCGTGGACA TCTCATCCAG 241 AAAGTGGGTG GGAAGAGGTG AGTGGCTACG ATGAGGCCAT GAATCCCATC CGCACATACC 301 AGGTGTGTAA TGTGCGCGAG TCAAGCCAGA ACAACTGGCT TCGCACGGGG TTCATCTGGC 361 GGCGGGATGT GCAGCGGGTC TACGTGGAGC TCAAGTTCAC TGTGCGTGAC TGCAACAGCA 421 TCCCCAACAT CCCCGGCTCC TGCAAGGAGA CCTTCAACCT CTTCTACTAC GAGGCTGACA 481 GCGATGTGGC CTCAGCCTCC TCCCCCTTCT GGATGGAGAA CCCCTACGTG AAAGTGGACA 541 CCATTGCACC CGATGAGAGC TTCTCGCGGC TGGATGCCGG CCGTGTCAAC ACCAAGGTGC 601 GCAGCTTTGG GCCACTTTCC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC CAGGGCGCCT 661 GCATGTCGCT CATCTCCGTG CGCGCCTTCT ACAAGAAGTG TGCATCCACC ACCGCAGGCT 721 TCGCACTCTT CCCCGAGACC CTCACTGGGG CGGAGCCCAC CTCGCTGGTC ATTGCTCCTG 781 GCACCTGCAT CCCTAACGCC GTGGAGGTGT CGGTGCCACT CAAGCTCTAC TGCAACGGCG 841 ATGGGGAGTG GATGGTGCCT GTGGGTGCCT GCACCTGTGC CACCGGCCAT GAGCCAGCTG 901 CCAAGGAGTC CCAGTGCCGC CCCTGTCCCC CTGGGAGCTA CAAGGCGAAG CAGGGAGAGG 961 GGCCCTGCCT CCCATGTCCC CCCAACAGCC GTACCACCTC CCCAGCCGCC AGCATCTGCA 1021 CCTGCCACAA TAACTTCTAC CGTGCAGACT CGGACTCTGC GGACAGTGCC TGTACCACCG 1081 TGCCATCTCC ACCCCGAGGT GTGATCTCCA ATGTGAATGA AACCTCACTG ATCCTCGAGT 1141 GGAGTGAGCC CCGGGACCTG GGTGGCCGGG ATGACCTCCT GTACAATGTC ATCTGCAAGA 1201 AGTGCCATGG GGCTGGAGGG GCCTCAGCCT GCTCACGCTG TGATGACAAC GTGGAGTTTG 1261 TGCCTCGGCA GCTGGGCCTG ACGGAGCGCC GGGTCCACAT CAGCCATCTG CTGGCCCACA 1321 CGCGCTACAC CTTTGAGGTG CAGGCGGTCA ACGGTGTCTC GGGCAAGAGC CCTCTGCCGC 1381 CTCGTTATGC GGCCGTGAAT ATCACCACAA ACCAGGCTGC CCCGTCTGAA GTGCCCACAC 1441 TACGCCTGCA CAGCAGCTCA GGCAGCAGCC TCACCCTATC CTGGGCACCC CCAGAGCGGC 1501 CCAACGGAGT CATCCTGGAC TACGAGATGA AGTACTTTGA GAAGAGCGAG GGCATCGCCT 1561 CCACAGTGAC CAGCCAGATG AACTCCGTGC AGCTGGACGG GCTTCGGCCT GACGCCCGCT 1621 ATGTGGTCCA GGTCCGTGCC CGCACAGTAG CTGGCTATGG GCAGTACAGC CGCCCTGCCG 1681 AGTTTGAGAC CACAAGTGAG AGAGGCTCTG GGGCCCAGCA GCTCCAGGAG CAGCTTCCCC 1741 TCATCGTGGG CTCCGCTACA GCTGGGCTTG TCTTCGTGGT GGCTGTCGTG GTCATCGCTA 1801 TCGTCTGCCT CAGGAAGCAG CGACACGGCT CTGATTCGGA GTACACGGAG AAGCTGCAGC 1861 AGTACATTGC TCCTGGAATG AAGGTTTATA TTGACCCTTT TACCTACGAG GACCCTAATG 1921 AGGCTGTTCG GGAGTTTGCC AAGGAGATCG ACGTGTCCTG CGTCAAGATC GAGGAGGTGA 1981 TCGGAGCTGG GGAATTTGGG GAAGTGTGCC GTGGTCGACT GAAACAGCCT GGCCGCCGAG 2041 AGGTGTTTGT GGCCATCAAG ACGCTGAAGG TGGGCTACAC CGAGAGGCAG CGGCGGGACT 2101 TCCTAAGCGA GGCCTCCATC ATGGGTCAGT TTGATCACCC CAATATAATC CGGCTCGAGG 2161 GCGTGGTCAC CAAAAGTCGG CCAGTTATGA TCCTCACTGA GTTCATGGAA AACTGCGCCC 2221 TGGACTCCTT CCTCCGGCTC AACGATGGGC AGTTCACGGT CATCCAGCTG GTGGGCATGT 2281 TGCGGGGCAT TGCTGCCGGC ATGAAGTACC TGTCCGAGAT GAACTATGTG CACCGCGACC 2341 TGGCTGCTCG CAACATCCTT GTCAACAGCA ACCTGGTCTG CAAAGTCTCA GACTTTGGCC 2401 TCTCCCGCTT CCTGGAGGAT GACCCCTCCG ATCCTACCTA CACCAGTTCC CTGGGCGGGA 2461 AGATCCCCAT CCGCTGGACT GCCCCAGAGG CCATAGCCTA TCGGAAGTTC ACTTCTGCTA 2521 GTGATGTCTG GAGCTACGGA ATTGTCATGT GGGAGGTCAT GAGCTATGGA GAGCGACCCT 2581 ACTGGGACAT GAGCAACCAG GATGTCATCA ATGCCGTGGA GCAGGATTAC CGGCTGCCAC 2641 CACCCATGGA CTGTCCCACA GCACTGCACC AGCTCATGCT GGACTGCTGG GTGCGGGACC 2701 GGAACCTCAG GCCCAAATTC TCCCAGATTG TCAATACCCT GGACAAGCTC ATCCGCAATG 2761 CTGCCAGCCT CAAGGTCATT GCCAGCGCTC AGTCTGGCAT GTCACAGCCC CTCCTGGACC 2821 GCACGGTCCC AGATTACACA ACCTTCACGA CAGTTGGTGA TTGGCTGGAT GCCATCAAGA 2881 TGGGGCGGTA CAAGGAGAGC TTCGTCAGTG CGGGGTTTGC ATCTTTTGAC CTGGTGGCCC 2941 AGATGACGGC AGAAGACCTG CTCCGTATTG GGGTCACCCT GGCCGGCCAC CAGAAGAAGA 3001 TCCTGAGCAG TATCCAGGAC ATGCGGCTGC AGATGAACCA GACGCTGCCT GTGCAGGTCT 3061 GCCCAACTTT CTTGTACAAA GTTGGcatta taagaaagca ttgcttatca atttgttgca 3121 acgaac