Construct: ORF ccsbBroadEn_00990
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000284.1_s300c1, BRDN0000388210
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJB9 (4189)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4189 | DNAJB9 | DnaJ heat shock protein fam... | NM_012328.3 | 100% | 100% | |
| 2 | mouse | 27362 | Dnajb9 | DnaJ heat shock protein fam... | NM_013760.4 | 91% | 93.7% | (many diffs) |
| 3 | mouse | 625253 | Gm6568 | predicted pseudogene 6568; ... | NR_040420.2 | 64.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC TACTCCCCAG TCAATTTTCA TCTTTGCAAT CTGCATTTTA ATGATAACAG 121 AATTAATTCT GGCCTCAAAA AGCTACTATG ATATCTTAGG TGTGCCAAAA TCGGCATCAG 181 AGCGCCAAAT CAAGAAGGCC TTTCACAAGT TGGCCATGAA GTACCACCCT GACAAAAATA 241 AGAGCCCGGA TGCTGAAGCA AAATTCAGAG AGATTGCAGA AGCATATGAA ACACTCTCAG 301 ATGCTAATAG ACGAAAAGAG TATGATACAC TTGGACACAG TGCTTTTACT AGTGGTAAAG 361 GACAAAGAGG TAGTGGAAGT TCTTTTGAGC AGTCATTTAA CTTCAATTTT GATGACTTAT 421 TTAAAGACTT TGGCTTTTTT GGTCAAAACC AAAACACTGG ATCCAAGAAG CGTTTTGAAA 481 ATCATTTCCA GACACGCCAG GATGGTGGTT CCAGTAGACA AAGGCATCAT TTCCAAGAAT 541 TTTCTTTTGG AGGTGGATTA TTTGATGACA TGTTTGAAGA TATGGAGAAA ATGTTTTCTT 601 TTAGTGGTTT TGACTCTACC AATCAGCATA CAGTACAGAC TGAAAATAGA TTTCATGGAT 661 CTAGCAAGCA CTGCAGGACT GTCACTCAAC GAAGAGGAAA TATGGTTACT ACATACACTG 721 ACTGTTCAGG ACAGTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 781 tatcaatttg ttgcaacgaa c