Construct: ORF ccsbBroadEn_01758
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017684.1_s300c1, BRDN0000384953
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UQCRH (7388)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_006004.4 | 100% | 100% | |
| 2 | human | 440567 | UQCRHL | ubiquinol-cytochrome c redu... | NM_001089591.1 | 98.5% | 96.7% | (many diffs) |
| 3 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_001297566.2 | 90.1% | 90.1% | 53_54ins27 |
| 4 | human | 7388 | UQCRH | ubiquinol-cytochrome c redu... | NM_001297565.2 | 77.2% | 60.3% | (many diffs) |
| 5 | mouse | 66576 | Uqcrh | ubiquinol-cytochrome c redu... | NM_025641.3 | 83.1% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG ACTGGAGGAC GAGCAAAAGA TGCTTACCGA ATCCGGAGAT CCTGAGGAGG 121 AGGAAGAGGA AGAGGAGGAA TTAGTGGATC CCCTAACAAC AGTGAGAGAG CAATGCGAGC 181 AGTTGGAGAA ATGTGTAAAG GCCCGGGAGC GGCTAGAGCT CTGTGATGAG CGTGTATCCT 241 CTCGATCACA TACAGAAGAG GATTGCACGG AGGAGCTCTT TGACTTCTTG CATGCGAGGG 301 ACCATTGCGT GGCCCACAAA CTCTTTAACA ACTTGAAATA CCCAACTTTC TTGTACAAAG 361 Ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaac