Construct: ORF ccsbBroadEn_02196
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011236.1_s300c1, BRDN0000388559
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPL33 (9553)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9553 | MRPL33 | mitochondrial ribosomal pro... | NM_004891.4 | 100% | 100% | |
| 2 | human | 9553 | MRPL33 | mitochondrial ribosomal pro... | NM_145330.2 | 32.7% | 32% | 41_42ins107;89_162del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 261
- ORF length:
- 195
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGTT CCTCTCCGCG GTCTTCTTTG CCAAGAGCAA GTCAAAAAAC ATTCTGGTGA 121 GAATGGTGAG CGAAGCTGGG ACAGGTTTCT GCTTCAACAC CAAGAGAAAC CGACTGCGGG 181 AAAAACTGAC TCTTTTGCAT TATGATCCAG TTGTGAAACA AAGAGTCCTC TTCGTGGAAA 241 AGAAAAAAAT ACGCTCCCTT TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc 301 attgcttatc aatttgttgc aacgaac