Construct: ORF ccsbBroadEn_03360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001011.1_s300c1, BRDN0000392601
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB1 (51665)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51665 | ASB1 | ankyrin repeat and SOCS box... | NM_001040445.3 | 100% | 100% | |
| 2 | human | 51665 | ASB1 | ankyrin repeat and SOCS box... | NM_001330196.2 | 69.8% | 69.8% | 189_190ins303 |
| 3 | mouse | 65247 | Asb1 | ankyrin repeat and SOCS box... | NM_001039126.2 | 86.8% | 93.7% | (many diffs) |
| 4 | mouse | 65247 | Asb1 | ankyrin repeat and SOCS box... | NM_023046.4 | 84.2% | 90.7% | (many diffs) |
| 5 | mouse | 65247 | Asb1 | ankyrin repeat and SOCS box... | XM_006529789.1 | 83.9% | 90.2% | (many diffs) |
| 6 | mouse | 65247 | Asb1 | ankyrin repeat and SOCS box... | XM_006529790.3 | 60.8% | 64.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccCTTTTTG TACAAAAAAG 61 TTGGCATGGC GGAGGGCGGC AGCCCAGACG GGCGGGCAGG GCCGGGCTCC GCAGGTCGTA 121 ATCTGAAGGA GTGGCTGAGG GAGCAATTTT GTGATCATCC GCTGGAGCAC TGTGAGGACA 181 CGAGGCTCCA TGATGCAGCT TACGTCGGGG ACCTCCAGAC CCTCAGGAGC CTATTGCAAG 241 AGGAGAGCTA CCGGAGCCGC ATCAACGAGA AGTCTGTCTG GTGCTGTGGC TGGCTCCCCT 301 GCACACCGTT GCGAATCGCG GCCACTGCAG GCCATGGGAG CTGTGTGGAC TTCCTCATCC 361 GGAAGGGGGC CGAGGTGGAT CTGGTGGACG TAAAAGGACA GACGGCCCTG TATGTGGCTG 421 TGGTGAACGG GCACCTAGAG AGTACCCAGA TCCTTCTCGA AGCTGGCGCG GACCCCAACG 481 GAAGCCGGCA CCATCGCAGC ACCCCTGTCT ACCACGCCTC TCGCGTGGGC CGGGCAGACA 541 TCCTGAAGGC CCTCATCAGG TACGGGGCTG ATGTTGACGT CAACCACCAC CTGACTCCTG 601 ATGTCCAGCC TCGATTCTCC CGGCGGCTCA CCTCCTTGGT GGTCTGCCCC TTGTACATCA 661 GCGCAGCCTA CCACAACCTC CAGTGCTTCC GGCTGCTCCT CCTGGCTGGC GCGAACCCTG 721 ACTTCAACTG CAATGGTCCT GTCAACACAC AGGGATTCTA CAGGGGCTCC CCTGGGTGCG 781 TCATGGATGC TGTTCTGCGC CACGGCTGTG AGGCAGCCTT CGTGAGCCTG CTGGTAGAAT 841 TTGGAGCCAA CCTGAATCTA GTGAAGTGGG AATCGCTGGG CCCAGAGTCG AGAGGAAGAA 901 GAAAAGTGGA CCCTGAGGCC TTGCAGGTCT TTAAAGAGGC CAGAAGTGTT CCCAGAACCT 961 TGCTGTGTCT GTGCCGTGTG GCTGTGAGAA GAGCTCTTGG CAAACACCGG CTTCATCTGA 1021 TTCCTTCGCT GCCTCTGCCA GACCCCATAA AGAAGTTTCT ACTCCATGAG TACCCAACTT 1081 TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac