Construct: ORF ccsbBroadEn_04645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002016.1_s300c1, BRDN0000388722
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C12orf57 (113246)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301834.1 | 100% | 100% | |
| 2 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_138425.4 | 100% | 100% | |
| 3 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301836.1 | 86.1% | 85.7% | (many diffs) |
| 4 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301837.1 | 76.9% | 76.9% | 141_142ins87 |
| 5 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NM_001301838.1 | 72.2% | 72.2% | 0_1ins105 |
| 6 | human | 113246 | C12orf57 | chromosome 12 open reading ... | NR_126035.1 | 32.6% | (many diffs) | |
| 7 | mouse | 14790 | Grcc10 | gene rich cluster, C10 gene | NM_013535.1 | 88.6% | 97.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 444
- ORF length:
- 378
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GTCCGCCTCG ACCCAACCGG CGGCCTTGAG CGCTGAGCAA GCAAAGGTGG 121 TCCTCGCGGA GGTGATCCAG GCGTTCTCCG CCCCGGAGAA TGCAGTGCGC ATGGACGAGG 181 CTCGGGATAA CGCCTGCAAC GACATGGGTA AGATGCTGCA ATTCGTGCTG CCCGTGGCCA 241 CGCAGATCCA GCAGGAGGTT ATCAAAGCCT ATGGCTTCAG CTGCGACGGG GAAGGTGTCC 301 TTAAGTTTGC TCGCTTGGTC AAGTCCTACG AAGCCCAGGA TCCTGAGATC GCCAGCCTGT 361 CAGGCAAGCT GAAGGCGCTG TTTCTGCCGC CCATGACCCT GCCACCCCAT GGGCCTGCTG 421 CTGGTGGCAG CGTGGCCGCC TCCTGCCCAA CTTTCTTGTA CAAAGTtggc attataagaa 481 agcattgctt atcaatttgt tgcaacgaac