Construct: ORF ccsbBroadEn_05997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005758.1_s300c1, BRDN0000389618
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHRM1 (1128)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1128 | CHRM1 | cholinergic receptor muscar... | NM_000738.3 | 99.9% | 99.7% | 1369T>A |
| 2 | human | 1128 | CHRM1 | cholinergic receptor muscar... | XM_011544742.2 | 99.9% | 99.7% | 1369T>A |
| 3 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_001112697.1 | 91.3% | 98.6% | (many diffs) |
| 4 | mouse | 12669 | Chrm1 | cholinergic receptor, musca... | NM_007698.3 | 91.3% | 98.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1446
- ORF length:
- 1380
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAA CACTTCAGCC CCACCTGCTG TCAGCCCCAA CATCACCGTC CTGGCACCAG 121 GAAAGGGTCC CTGGCAAGTG GCCTTCATTG GGATCACCAC GGGCCTCCTG TCGCTAGCCA 181 CAGTGACAGG CAACCTGCTG GTACTCATCT CTTTCAAGGT CAACACGGAG CTCAAGACAG 241 TCAATAACTA CTTCCTGCTG AGCCTGGCCT GTGCTGACCT CATCATCGGT ACCTTCTCCA 301 TGAACCTCTA TACCACGTAC CTGCTCATGG GCCACTGGGC TCTGGGCACG CTGGCTTGTG 361 ACCTCTGGCT GGCCCTGGAC TATGTGGCCA GCAATGCCTC CGTCATGAAT CTGCTGCTCA 421 TCAGCTTTGA CCGCTACTTC TCCGTGACTC GGCCCCTGAG CTACCGTGCC AAGCGCACAC 481 CCCGCCGGGC AGCTCTGATG ATCGGCCTGG CCTGGCTGGT TTCCTTTGTG CTCTGGGCCC 541 CAGCCATCCT CTTCTGGCAG TACCTGGTAG GGGAGCGGAC AGTGCTAGCT GGGCAGTGCT 601 ACATCCAGTT CCTCTCCCAG CCCATCATCA CCTTTGGCAC AGCCATGGCT GCCTTCTACC 661 TCCCTGTCAC AGTCATGTGC ACGCTCTACT GGCGCATCTA CCGGGAGACA GAGAACCGAG 721 CACGGGAGCT GGCAGCCCTT CAGGGCTCCG AGACGCCAGG CAAAGGGGGT GGCAGCAGCA 781 GCAGCTCAGA GAGGTCTCAG CCAGGGGCTG AGGGCTCACC AGAGACTCCT CCAGGCCGCT 841 GCTGTCGCTG CTGCCGGGCC CCCAGGCTGC TGCAGGCCTA CAGCTGGAAG GAAGAAGAGG 901 AAGAGGACGA AGGCTCCATG GAGTCCCTCA CATCCTCAGA GGGAGAGGAG CCTGGCTCCG 961 AAGTGGTGAT CAAGATGCCA ATGGTGGACC CCGAGGCACA GGCCCCCACC AAGCAGCCCC 1021 CACGGAGCTC CCCAAATACA GTCAAGAGGC CGACTAAGAA AGGGCGTGAT CGAGCTGGCA 1081 AGGGCCAGAA GCCCCGTGGA AAGGAGCAGC TGGCCAAGCG GAAGACCTTC TCGCTGGTCA 1141 AGGAGAAGAA GGCGGCTCGG ACCCTGAGTG CCATCCTCCT GGCCTTCATC CTCACCTGGA 1201 CACCGTACAA CATCATGGTG CTGGTGTCCA CCTTCTGCAA GGACTGTGTT CCCGAGACCC 1261 TGTGGGAGCT GGGCTACTGG CTGTGCTACG TCAACAGCAC CATCAACCCC ATGTGCTACG 1321 CACTCTGCAA CAAAGCCTTC CGGGACACCT TTCGCCTGCT GCTGCTTTGC CGCTGGGACA 1381 AGAGACGCTG GCGCAAGATC CCCAAGCGCC CTGGCTCCGT GCACCGCACT CCCACCCGCC 1441 AATGCTGCCC AACTTTCTTG TACAAAGTtg gcattataag aaagcattgc ttatcaattt 1501 gttgcaacga ac