Construct: ORF ccsbBroadEn_07809

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013226.1_s300c1, BRDN0000456931
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ATG14 (22863)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22863 ATG14 autophagy related 14 NM_014924.5 99.9% 99.7% 767A>G
2 human 22863 ATG14 autophagy related 14 XM_011536563.2 76.9% 76.8% 0_1ins339;428A>G
3 mouse 100504663 Atg14 autophagy related 14 NM_172599.4 86.8% 92.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1545
ORF length:
1476
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG
61TTGGCACCAT GGCGTCTCCC AGTGGGAAGG GAGCCCGGGC GCTGGAGGCT CCTGGCTGCG
121GGCCCCGGCC GCTCGCCCGG GACCTGGTGG ACTCCGTGGA CGATGCGGAG GGGCTGTACG
181TGGCTGTGGA GCGCTGCCCG CTGTGCAACA CTACCCGCCG GCGGCTGACC TGCGCCAAAT
241GCGTTCAGAG CGGCGATTTC GTCTACTTCG ACGGCCGCGA CCGGGAGAGG TTTATCGACA
301AGAAGGAAAG GTTAAGCCGA CTTAAGAGCA AGCAAGAAGA ATTTCAGAAA GAAGTGTTAA
361AAGCTATGGA AGGAAAATGG ATAACAGATC AGTTGAGATG GAAAATAATG TCCTGCAAGA
421TGAGGATTGA ACAGTTAAAA CAAACAATAT GTAAAGGAAA TGAAGAAATG GAGAAAAATT
481CTGAAGGCCT TCTCAAAACC AAGGAAAAGA ATCAGAAGCT TTACAGTCGA GCACAACGGC
541ACCAAGAGAA AAAGGAGAAG ATTCAGAGGC ATAATCGCAA ACTTGGTGAC CTGGTAGAAA
601AAAAGACCAT TGACTTAAGA AGTCATTATG AGCGTCTGGC AAATCTTCGA CGATCCCATA
661TATTAGAGCT CACCTCTGTC ATTTTTCCAA TCGAGGAAGT AAAGACGGGT GTGAGAGACC
721CCGCAGATGT GTCTTCAGAG AGTGACAGTG CCATGACCTC CAGCACTGTG AGCAAGCTTG
781CTGAAGCCCG GAGGACAACT TACCTCTCAG GACGATGGGT CTGTGACGAT CACAGCGGAG
841ACACCAGCAT TAGCATTACA GGGCCTTGGA TTAGCCTCCC TAACAATGGG GACTACTCTG
901CCTACTACAG CTGGGTGGAG GAGAAGAAAA CAACCCAGGG GCCTGACATG GAGCAGAGTA
961ACCCTGCCTA CACCATCAGT GCTGCGCTGT GCTATGCAAC TCAGCTGGTC AACATTCTGT
1021CTCATATACT TGATGTAAAT CTTCCCAAAA AGCTCTGCAA CAGTGAATTT TGTGGCGAAA
1081ATCTAAGCAA GCAGAAATTT ACTCGAGCAG TGAAGAAACT GAATGCAAAT ATTCTTTACC
1141TTTGTTTTTC TCAGCATGTA AATTTAGATC AATTACAACC ACTGCATACC CTCAGGAATC
1201TAATGTACCT GGTCAGTCCA AGCTCTGAAC ACCTAGGCAG GTCAGGGCCC TTTGAAGTAC
1261GAGCAGACCT TGAGGAGTCC ATGGAATTTG TGGATCCCGG AGTTGCTGGA GAATCAGATG
1321AGAGCGGAGA TGAGCGCGTC AGCGATGAAG AAACCGACCT GGGCACAGAC TGGGAGAACT
1381TGCCTAGTCC CCGGTTTTGT GATATCCCTT CCCAGTCTGT GGAAGTCTCC CAGAGTCAGA
1441GCACCCAGGC GTCCCCACCC ATCGCGAGCA GCAGTGCAGG TGGGATGATC TCCTCTGCAG
1501CAGCCTCGGT GACCTCCTGG TTTAAAGCTT ACACTGGACA CCGTTTGCCA ACTTTCTTGT
1561ACAAAGTTGG cattataaga aagcattgct tatcaatttg ttgcaacgaa c

Download FASTA (ORF) (Full)