Construct: ORF ccsbBroadEn_07916
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001803.1_s300c1, BRDN0000456221
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSSK2 (23617)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23617 | TSSK2 | testis specific serine kina... | NM_053006.5 | 99.8% | 99.4% | 438G>T;911G>A |
| 2 | mouse | 22115 | Tssk2 | testis-specific serine kina... | NM_009436.2 | 89.5% | 91.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1143
- ORF length:
- 1074
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGACGATGCC ACAGTCCTAA GGAAGAAGGG TTACATCGTA GGCATCAATC 121 TTGGCAAGGG TTCCTACGCA AAAGTCAAAT CTGCCTACTC TGAGCGCCTC AAGTTCAATG 181 TGGCTGTCAA GATCATCGAC CGCAAGAAAA CACCTACTGA CTTTGTGGAG AGATTCCTTC 241 CTCGGGAGAT GGACATCCTG GCAACTGTCA ACCACGGCTC CATCATCAAG ACTTACGAGA 301 TCTTTGAGAC CTCTGACGGA CGGATCTACA TCATCATGGA GCTTGGCGTC CAGGGCGACC 361 TCCTCGAGTT CATCAAGTGC CAGGGAGCCC TGCATGAGGA CGTGGCACGC AAGATGTTCC 421 GACAGCTCTC CTCCGCCGTC AAGTACTGCC ACGACCTGGA CATCGTCCAC CGGGACCTCA 481 AGTGCGAGAA CCTTCTCCTC GACAATGACT TCAACATCAA GCTGTCTGAC TTTGGCTTCT 541 CCAAGCGCTG CCTGCGGGAC AGCAATGGGC GCATCATCCT CAGCAAGACC TTCTGCGGGT 601 CGGCAGCATA TGCAGCCCCC GAGGTGCTGC AGAGCATCCC CTACCAGCCC AAGGTGTATG 661 ACATCTGGAG CCTGGGCGTG ATCCTGTACA TCATGGTCTG CGGCTCCATG CCCTATGACG 721 ACTCCGACAT CAGGAAGATG CTGCGTATCC AGAAGGAGCA CCGTGTGGAC TTCCCGCGCT 781 CCAAGAACCT GACCTGCGAG TGCAAGGACC TCATCTACCG CATGCTGCAG CCCGACGTCA 841 GCCAGCGGCT CCACATCGAT GAGATCCTCA GCCACTCGTG GCTGCAGCCC CCCAAGCCCA 901 AAGCCACGTC TTCTGCCTCC TTCAAGAGGG AGGGGGAGGG CAAGTACCGC GCTGAGTGCA 961 AACTGGACAC CAAGACAGAC TTGAGGCCCG ACCACCGGCC CGACCACAAG CTTGGAGCCA 1021 AAACCCAGCA CCGGCTGCTG GTGGTGCCCG AGAACGAGAA CAGGATGGAG GACAGGCTGG 1081 CCGAGACCTC CAGGGCCAAA GACCATCACA TCTCCGGAGC TGAGGTGGGG AAAGCAAGCA 1141 CCTTGCCAAC TTTCTTGTAC AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt 1201 gcaacgaac