Construct: ORF ccsbBroadEn_08128

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006586.1_s300c1, BRDN0000385817
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG5 (29880)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29880 ALG5 ALG5 dolichyl-phosphate bet... NM_013338.5 99.8% 100% 6T>C
2 human 29880 ALG5 ALG5 dolichyl-phosphate bet... NM_001142364.1 90.6% 90.7% 6T>C;282_283ins90
3 human 29880 ALG5 ALG5 dolichyl-phosphate bet... XM_017020552.1 80.5% 72.6% 0_1ins142;96_97ins47
4 human 29880 ALG5 ALG5 dolichyl-phosphate bet... XR_001749540.2 76.6% (many diffs)
5 mouse 66248 Alg5 asparagine-linked glycosyla... NM_025442.3 86.1% 89.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1038
ORF length:
972
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGC CCCGCTTCTG TTGCAGCTGG CGGTGCTCGG CGCGGCGCTG GCGGCCGCAG
121CCCTCGTACT GATTTCCATC GTTGCATTTA CAACTGCTAC AAAAATGCCA GCACTCCATC
181GACATGAAGA AGAGAAATTC TTCTTAAATG CCAAAGGCCA GAAAGAAACT TTACCCAGCA
241TATGGGACTC ACCTACCAAA CAACTTTCTG TCGTTGTGCC TTCATACAAT GAAGAAAAAC
301GGTTGCCTGT GATGATGGAT GAAGCTCTGA GCTATCTAGA GAAGAGACAG AAACGAGATC
361CTGCGTTCAC TTATGAAGTG ATAGTAGTTG ATGATGGCAG TAAAGATCAG ACCTCAAAGG
421TAGCTTTTAA ATATTGCCAG AAATATGGAA GTGACAAAGT ACGTGTGATA ACCCTGGTGA
481AGAATCGTGG AAAAGGTGGA GCGATTAGAA TGGGTATATT CAGTTCTCGA GGAGAAAAGA
541TCCTTATGGC AGATGCTGAT GGAGCCACAA AGTTTCCAGA TGTTGAGAAA TTAGAAAAGG
601GGCTAAATGA TCTACAGCCT TGGCCTAATC AAATGGCTAT AGCATGTGGA TCTCGAGCTC
661ATTTAGAAAA AGAATCAATT GCTCAGCGTT CTTACTTCCG TACTCTTCTC ATGTATGGGT
721TCCACTTTCT GGTGTGGTTC CTTTGTGTCA AAGGAATCAG GGACACACAG TGTGGGTTCA
781AATTATTTAC TCGAGAAGCA GCTTCACGGA CGTTTTCATC TCTACACGTT GAACGATGGG
841CATTTGATGT AGAACTACTG TACATAGCAC AGTTCTTTAA AATTCCAATA GCAGAAATTG
901CTGTCAACTG GACAGAAATT GAAGGTTCTA AATTAGTTCC ATTCTGGAGC TGGCTACAAA
961TGGGTAAAGA CCTACTTTTT ATACGACTTC GATATTTGAC TGGTGCCTGG AGGCTTGAGC
1021AAACTCGGAA AATGAATTAC CCAACTTTCT TGTACAAAGT tggcattata agaaagcatt
1081gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)