Construct: ORF ccsbBroadEn_08246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001089.1_s300c1, BRDN0000457405
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HERC5 (51191)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51191 | HERC5 | HECT and RLD domain contain... | NM_016323.4 | 99.9% | 99.9% | 303C>A;1492A>G;2358T>C |
| 2 | human | 51191 | HERC5 | HECT and RLD domain contain... | XM_011532022.2 | 80.6% | 80.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3141
- ORF length:
- 3072
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GGAGCGGAGG TCGCGGAGGA AGTCGCGGCG CAACGGGCGC TCGACCGCGG 121 GCAAGGCCGC CGCGACCCAG CCCGCGAAGT CTCCGGGCGC ACAGCTCTGG CTCTTTCCCA 181 GCGCCGCGGG CCTCCACCGC GCGCTGCTCC GGAGGGTGGA GGTGACGCGC CAACTCTGCT 241 GCTCGCCGGG GCGCCTCGCG GTCTTGGAAC GCGGCGGGGC GGGCGTCCAG GTTCACCAGC 301 TGCTCGCCGG GAGCGGCGGC GCCCGGACGC CGAAATGCAT TAAATTAGGA AAAAACATGA 361 AGATACATTC AGTGGACCAA GGAGCAGAGC ACATGCTGAT TCTCTCATCA GATGGAAAAC 421 CATTTGAGTA TGACAACTAT AGCATGAAAC ATCTAAGGTT TGAAAGCATT TTACAAGAAA 481 AAAAAATAAT TCAGATCACA TGTGGAGATT ACCATTCTCT TGCACTCTCA AAAGGTGGTG 541 AGCTTTTTGC CTGGGGACAG AACCTGCATG GGCAGCTTGG AGTTGGAAGG AAATTTCCCT 601 CAACCACCAC ACCACAGATT GTGGAGCACC TCGCAGGAGT ACCCTTGGCT CAGATTTCTG 661 CCGGAGAAGC CCACAGCATG GCCTTATCCA TGTCTGGCAA CATTTATTCA TGGGGAAAAA 721 ATGAATGTGG ACAACTAGGC CTGGGCCACA CTGAGAGTAA AGATGATCCA TCCCTTATTG 781 AAGGACTAGA CAATCAGAAA GTTGAATTTG TCGCTTGTGG TGGCTCTCAC AGTGCCCTAC 841 TCACACAGGA TGGGCTGCTG TTTACTTTCG GTGCTGGAAA ACATGGGCAA CTTGGTCATA 901 ATTCAACACA GAATGAGCTA AGACCCTGTT TGGTGGCTGA GCTTGTTGGG TATAGAGTGA 961 CTCAGATAGC ATGTGGAAGG TGGCACACAC TTGCCTATGT TTCTGATTTG GGAAAGGTCT 1021 TTTCCTTTGG TTCTGGAAAA GATGGACAAC TGGGAAATGG TGGAACACGT GACCAGCTGA 1081 TGCCGCTTCC AGTGAAAGTA TCATCAAGTG AAGAACTCAA ACTTGAAAGC CATACCTCAG 1141 AAAAGGAGTT AATAATGATT GCTGGAGGGA ATCAAAGCAT TTTGCTCTGG ATAAAGAAAG 1201 AGAATTCATA TGTTAATCTG AAGAGGACAA TTCCTACTCT GAATGAAGGG ACTGTAAAGA 1261 GATGGATTGC TGATGTGGAG ACTAAACGGT GGCAGAGCAC AAAAAGGGAA ATCCAAGAGA 1321 TATTTTCATC TCCTGCTTGT CTAACTGGAA GTTTTTTAAG GAAAAGAAGA ACTACAGAAA 1381 TGATGCCTGT TTATTTGGAC TTAAATAAAG CAAGAAACAT CTTCAAGGAG TTAACCCAAA 1441 AGGACTGGAT TACTAACATG ATAACCACCT GCCTCAAAGA TAATCTGCTC AAAAGACTTC 1501 CATTTCATTC TCCACCCCAA GAAGCTTTAG AAATTTTCTT CCTTCTCCCA GAATGTCCTG 1561 TGATGCATAT TTCCAACAAC TGGGAGAGCC TTGTGGTTCC ATTTGCAAAG GTTGTTTGTA 1621 AAATGAGTGA CCAGTCTTCA CTGGTTCTGG AAGAGTATTG GGCAACTCTG CAAGAATCCA 1681 CTTTCAGCAA ACTGGTCCAG ATGTTTAAAA CAGCCGTCAT ATGCCAGTTG GATTACTGGG 1741 ATGAAAGTGC TGAGGAGAAT GGTAATGTTC AAGCTCTCCT AGAAATGTTG AAGAAGCTGC 1801 ACAGGGTAAA CCAGGTGAAA TGTCAACTAC CTGAAAGTAT TTTCCAAGTA GACGAACTCT 1861 TGCACCGTCT CAATTTTTTT GTAGAAGTAT GCAGAAGGTA CTTGTGGAAA ATGACTGTGG 1921 ACGCTTCAGA AAATGTACAA TGCTGCGTCA TATTCAGTCA CTTTCCATTT ATCTTTAATA 1981 ATCTGTCGAA AATTAAACTA CTACATACAG ACACACTTTT AAAAATAGAG AGTAAAAAAC 2041 ATAAAGCTTA TCTTAGGTCG GCAGCAATTG AGGAAGAAAG AGAGTCTGAA TTCGCTTTGA 2101 GGCCCACGTT TGATCTAACA GTCAGAAGGA ATCACTTGAT TGAGGATGTT TTGAATCAGC 2161 TAAGTCAATT TGAGAATGAA GACCTGAGGA AAGAGTTATG GGTTTCATTT AGTGGAGAAA 2221 TTGGGTATGA CCTCGGAGGA GTCAAGAAAG AGTTCTTCTA CTGTCTGTTT GCAGAGATGA 2281 TCCAGCCGGA ATATGGGATG TTCATGTATC CTGAAGGGGC TTCCTGCATG TGGTTTCCTG 2341 TCAAGCCTAA ATTTGAGAAG AAAAGATACT TCTTTTTTGG GGTTCTATGT GGACTTTCCC 2401 TGTTCAATTG CAATGTTGCC AACCTCCCTT TCCCACTGGC ACTGTTTAAG AAACTTTTGG 2461 ACCAAATGCC ATCATTGGAA GACTTGAAAG AACTCAGTCC TGATTTGGGA AAGAATTTGC 2521 AAACACTTCT GGATGATGAA GGTGATAACT TTGAGGAAGT ATTTTACATC CATTTTAATG 2581 TGCACTGGGA CAGAAACGAC ACAAACTTAA TTCCTAATGG AAGTAGCATA ACTGTCAACC 2641 AGACTAACAA GAGAGACTAT GTTTCTAAGT ATATCAATTA CATTTTCAAC GACTCTGTAA 2701 AGGCGGTTTA TGAAGAATTT CGGAGAGGAT TTTATAAAAT GTGCGACGAA GACATTATCA 2761 AATTATTCCA CCCCGAAGAA CTGAAGGATG TGATTGTTGG AAATACAGAT TATGATTGGA 2821 AAACATTTGA AAAGAATGCA CGTTATGAAC CAGGATATAA CAGTTCACAT CCCACCATAG 2881 TGATGTTTTG GAAGGCTTTC CACAAATTGA CTCTGGAAGA AAAGAAAAAA TTCCTTGTAT 2941 TTCTTACAGG AACTGACAGA CTACAAATGA AAGATTTAAA TAATATGAAA ATAACATTTT 3001 GCTGTCCTGA AAGTTGGAAT GAAAGAGACC CTATAAGAGC ACTGACATGT TTCAGTGTCC 3061 TCTTCCTCCC TAAATATTCT ACAATGGAAA CAGTTGAAGA AGCGCTTCAA GAAGCCATCA 3121 ACAACAACAG AGGATTTGGC TTGCCAACTT TCTTGTACAA AGTTGGcatt ataagaaagc 3181 attgcttatc aatttgttgc aacgaac