Construct: ORF ccsbBroadEn_09490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007135.1_s300c1, BRDN0000389594
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIEF2 (125170)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125170 | MIEF2 | mitochondrial elongation fa... | NM_139162.4 | 99.9% | 100% | 792T>C |
| 2 | human | 125170 | MIEF2 | mitochondrial elongation fa... | XM_017024190.1 | 98.4% | 98.4% | 1_21del;813T>C |
| 3 | human | 125170 | MIEF2 | mitochondrial elongation fa... | NM_148886.1 | 97.5% | 97.6% | 1_33del;825T>C |
| 4 | human | 125170 | MIEF2 | mitochondrial elongation fa... | NM_001144900.2 | 45.1% | 26% | 307_308ins74;615_616ins673 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1428
- ORF length:
- 1362
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC AGAGTTCTCC CAGAAACGGG GGAAGCGGCG TAGCGACGAA GGGCTGGGCA 121 GCATGGTGGA CTTCCTCCTG GCCAATGCCC GCCTGGTGCT GGGCGTGGGC GGGGCTGCTG 181 TGCTGGGCAT TGCCACCCTG GCCGTGAAGC GGTTCATTGA CAGGGCCACT AGCCCGCGGG 241 ATGAGGATGA CACCAAGGCA GACAGCTGGA AGGAACTGAG CCTGCTCAAG GCCACACCAC 301 ACCTGCAGCC CCGGCCTCCA CCTGCTGCCC TTAGCCAGCC AGTGTTGCCC TTGGCCCCCT 361 CGTCGTCTGC CCCAGAAGGG CCTGCAGAAA CTGATCCTGA GGTGACACCA CAGCTCAGCT 421 CCCCAGCACC GCTGTGTCTG ACACTGCAGG AGAGGCTGCT GGCCTTCGAG CGGGACCGTG 481 TGACCATCCC AGCAGCCCAG GTGGCTTTGG CCAAACAGCT GGCTGGCGAC ATCGCCCTGG 541 AGCTGCAGGC CTACTTTCGG AGCAAGTTCC CGGAACTGCC CTTTGGGGCA TTCGTGCCTG 601 GGGGGCCGCT CTACGACGGG CTGCAGGCGG GGGCTGCGGA CCATGTGCGT CTCCTGGTGC 661 CACTGGTGCT GGAGCCGGGC CTGTGGAGCC TGGTGCCGGG CGTGGACACT GTGGCGAGGG 721 ACCCTCGCTG CTGGGCCGTG CGCAGGACGC AGCTTGAGTT CTGCCCCCGT GGGAGCAGCC 781 CCTGGGACCG CTTCCTGGTC GGGGGCTACC TCTCCTCCCG CGTCCTGCTG GAGCTACTCC 841 GCAAGGCGCT GGCTGCCTCT GTCAACTGGC CGGCCATTGG CAGCCTTCTC GGGTGCCTGA 901 TCCGGCCCAG CATGGCCTCG GAGGAGCTGC TGCTCGAGGT GCAGCACGAA CGCCTGGAGC 961 TCACTGTGGC TGTGCTTGTG GCAGTCCCTG GGGTCGATGC TGACGACCGC CTCCTCTTGG 1021 CCTGGCCCCT GGAGGGGCTG GCGGGGAACC TCTGGCTGCA GGACCTGTAT CCAGTGGAGG 1081 CTGCTAGGCT GCGAGCCCTG GACGACCATG ACGCTGGGAC TCGCCGGCGG CTGCTGCTGC 1141 TGCTGTGTGC TGTCTGCCGT GGTTGCTCGG CTCTGGGGCA GCTAGGCCGG GGTCACCTGA 1201 CCCAGGTGGT CCTGCGTCTG GGGGAGGACA ACGTGGATTG GACGGAGGAG GCCTTGGGTG 1261 AGCGCTTCCT GCAAGCCCTG GAGCTGCTCA TCGGCAGCCT GGAGCAGGCC AGCCTGCCCT 1321 GCCACTTCAA CCCCAGCGTG AACCTCTTCA GCAGCTTGCG TGAGGAGGAG ATTGACGACA 1381 TTGGCTATGC GCTATACAGT GGCCTACAGG AGCCCGAGGG GCTGCTCTAC CCAACTTTCT 1441 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac