Construct: ORF ccsbBroadEn_09559
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014972.1_s300c1, BRDN0000391821
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SDHAF4 (135154)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135154 | SDHAF4 | succinate dehydrogenase com... | NM_145267.3 | 99.6% | 99% | 155A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 390
- ORF length:
- 324
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAC CCCATCGAGG CTTCCCTGGT TGCTTAGCTG GGTCTCGGCC ACGGCGTGGA 121 GAGCGGCAAG ATCACCCCTT CTGTGTCATT CTCTGAGGAA AACAAGTTCT TCTCAAGGAG 181 GAAAGTCTGA ACTTGTCAAA CAGTCCCTTA AGAAGCCGAT GTTACCAGAA GGTCGTTTTG 241 ATGCACCAGA GGATTCCCAT TTAGAGAAAG AACCACTGGA AAAATTTCCA GATGATGTTA 301 ATCCAGTGAC CAAAGAAAAA GGTGGACCCA GGGGCCCAGA ACCTACCCGA TATGGAGATT 361 GGGAACGAAA AGGACGCTGT ATTGATTTTT ACCCAACTTT GTTGTACAAA GTtggcatta 421 taagaaagca ttgcttatca atttgttgca acgaac