Construct: ORF ccsbBroadEn_10200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011879.1_s300c1, BRDN0000456313
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCR2 (729230)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729230 | CCR2 | C-C motif chemokine receptor 2 | NM_001123041.2 | 99.8% | 100% | 780T>C;993G>A |
| 2 | human | 729230 | CCR2 | C-C motif chemokine receptor 2 | NM_001123396.3 | 89% | 84.3% | (many diffs) |
| 3 | human | 729230 | CCR2 | C-C motif chemokine receptor 2 | XM_011534069.1 | 89% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1191
- ORF length:
- 1122
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCTGTCCACA TCTCGTTCTC GGTTTATCAG AAATACCAAC GAGAGCGGTG 121 AAGAAGTCAC CACCTTTTTT GATTATGATT ACGGTGCTCC CTGTCATAAA TTTGACGTGA 181 AGCAAATTGG GGCCCAACTC CTGCCTCCGC TCTACTCGCT GGTGTTCATC TTTGGTTTTG 241 TGGGCAACAT GCTGGTCGTC CTCATCTTAA TAAACTGCAA AAAGCTGAAG TGCTTGACTG 301 ACATTTACCT GCTCAACCTG GCCATCTCTG ATCTGCTTTT TCTTATTACT CTCCCATTGT 361 GGGCTCACTC TGCTGCAAAT GAGTGGGTCT TTGGGAATGC AATGTGCAAA TTATTCACAG 421 GGCTGTATCA CATCGGTTAT TTTGGCGGAA TCTTCTTCAT CATCCTCCTG ACAATCGATA 481 GATACCTGGC TATTGTCCAT GCTGTGTTTG CTTTAAAAGC CAGGACGGTC ACCTTTGGGG 541 TGGTGACAAG TGTGATCACC TGGTTGGTGG CTGTGTTTGC TTCTGTCCCA GGAATCATCT 601 TTACTAAATG CCAGAAAGAA GATTCTGTTT ATGTCTGTGG CCCTTATTTT CCACGAGGAT 661 GGAATAATTT CCACACAATA ATGAGGAACA TTTTGGGGCT GGTCCTGCCG CTGCTCATCA 721 TGGTCATCTG CTACTCGGGA ATCCTGAAAA CCCTGCTTCG GTGTCGAAAC GAGAAGAAGA 781 GGCATAGGGC AGTGAGAGTC ATCTTCACCA TCATGATTGT TTACTTTCTC TTCTGGACTC 841 CCTATAACAT TGTCATTCTC CTGAACACCT TCCAGGAATT CTTCGGCCTG AGTAACTGTG 901 AAAGCACCAG TCAACTGGAC CAAGCCACGC AGGTGACAGA GACTCTTGGG ATGACTCACT 961 GCTGCATCAA TCCCATCATC TATGCCTTCG TTGGGGAGAA GTTCAGAAGC CTTTTTCACA 1021 TAGCTCTTGG CTGTAGGATT GCCCCACTCC AAAAACCAGT ATGTGGAGGT CCAGGAGTGA 1081 GACCAGGAAA GAATGTGAAA GTGACTACAC AAGGACTCCT CGATGGTCGT GGAAAAGGAA 1141 AGTCAATTGG CAGAGCCCCT GAAGCCAGTC TTCAGGACAA AGAAGGAGCC TTGCCAACTT 1201 TCTTGTACAA AGTTGGcatt ataagaaagc attgcttatc aatttgttgc aacgaac