Construct: ORF ccsbBroadEn_11541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008213.1_s300c1, BRDN0000455602
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERS1 (10715)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10715 | CERS1 | ceramide synthase 1 | NM_001290265.1 | 100% | 100% | |
| 2 | human | 10715 | CERS1 | ceramide synthase 1 | NM_198207.3 | 70.9% | 70.9% | 1_294del |
| 3 | human | 10715 | CERS1 | ceramide synthase 1 | NM_021267.5 | 68.2% | 68.2% | 1_294del;1012_1050del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 786
- ORF length:
- 717
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAAAAAG 61 TTGGCACCAT GCCCGAGAGC GCTTGGAAGT TTCTCTTCTA CCTGGGCAGC TGGAGCTACA 121 GTGCCTACCT GCTGTTTGGC ACCGACTACC CCTTCTTCCA TGACCCACCA TCTGTCTTCT 181 ACGACTGGAC GCCGGGCATG GCAGTGCCAC GGGACATTGC AGCCGCCTAC CTGCTCCAGG 241 GAAGCTTCTA TGGCCACTCC ATCTACGCTA CGCTATACAT GGACACCTGG CGCAAGGACT 301 CGGTGGTCAT GCTGCTCCAC CACGTGGTCA CTCTCATCCT CATCGTCTCC TCCTACGCCT 361 TCCGGTACCA CAATGTGGGC ATCCTTGTGC TCTTCCTGCA CGATATCAGT GACGTGCAGC 421 TTGAGTTCAC CAAGCTCAAC ATTTACTTCA AGTCCCGCGG CGGCTCCTAC CATCGGCTGC 481 ATGCCTTGGC AGCAGACTTG GGCTGCCTCA GCTTCGGCTT CAGCTGGTTC TGGTTCCGCC 541 TCTACTGGTT CCCGCTCAAG GTCCTGTATG CCACCAGTCA CTGCAGTCTG CGCACGGTGC 601 CTGACATCCC CTTCTACTTC TTCTTCAATG CGCTCCTGCT GCTGCTCACC CTTATGAACC 661 TCTACTGGTT CCTGTACATC GTGGCGTTTG CAGCCAAGGT GTTGACAGGC CAGGTGCACG 721 AGCTGAAGGA CCTGCGGGAG TATGACACAG CCGAGGCCCA GAGCCTGAAG CCCAGCAAAG 781 CCGAGTTGCC AACTTTCTTG TACAAAGTTG Gcattataag aaagcattgc ttatcaattt 841 gttgcaacga ac