Construct: ORF ccsbBroadEn_12405
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002655.1_s300c1, BRDN0000393382
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLD4 (57804)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NM_001256870.1 | 42.1% | 41.7% | 99_105delGGAGGAGinsT;107C>T;109_237del |
| 2 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NM_021173.5 | 31.1% | 30.8% | 99_105delGGAGGAGinsT;107C>T;109_321del |
| 3 | human | 57804 | POLD4 | DNA polymerase delta 4, acc... | NR_046411.1 | 5.7% | 1_197del;300_1760del | |
| 4 | mouse | 69745 | Pold4 | polymerase (DNA-directed), ... | NM_027196.4 | 28.9% | 28% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 168
- ORF length:
- 102
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG CCGGAAGCGG CTCATCACTG ATTCCTACCC GGTTGTGAAG AGGAGGGAGG 121 GGCCCGCTGG GCACAGCAAG GGGGAGCTGG CACCCGAGCT AGGTCTCTGC CCAACTTTCT 181 TGTACAAAGT tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac