Construct: ORF ccsbBroadEn_12634

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF016774.1_s300c1, BRDN0000390155
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: CSPP1 (79848)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	79848	CSPP1	centrosome and spindle pole...	XM_017013856.2	20.6%	20.4%	616_617delAA;627_3024del
2	human	79848	CSPP1	centrosome and spindle pole...	XM_024447282.1	20%	19.9%	302_382del;697_698delAA;708_3105del
3	human	79848	CSPP1	centrosome and spindle pole...	XM_017013855.2	20%	19.8%	1_246del;862_863delAA;873_3117del
4	human	79848	CSPP1	centrosome and spindle pole...	XM_017013854.2	18.6%	18.4%	(many diffs)
5	human	79848	CSPP1	centrosome and spindle pole...	XM_006716477.3	18.4%	18.3%	(many diffs)
6	human	79848	CSPP1	centrosome and spindle pole...	NM_001363133.2	18.3%	18.1%	616_617delAA;627_3402del
7	human	79848	CSPP1	centrosome and spindle pole...	XM_024447281.1	18.3%	18.1%	616_617delAA;627_3402del
8	human	79848	CSPP1	centrosome and spindle pole...	NM_001363132.2	17.9%	17.7%	302_382del;697_698delAA;708_3483del
9	human	79848	CSPP1	centrosome and spindle pole...	XM_017013852.2	17.5%	17.4%	1_246del;862_863delAA;873_3549del
10	human	79848	CSPP1	centrosome and spindle pole...	XM_017013851.2	17.5%	17.3%	616_617delAA;627_3555del
11	human	79848	CSPP1	centrosome and spindle pole...	NM_001364870.1	17.5%	17.3%	1_108del;724_725delAA;735_3564del
12	human	79848	CSPP1	centrosome and spindle pole...	NM_001363131.2	17.3%	17.1%	616_617delAA;627_3597del
13	human	79848	CSPP1	centrosome and spindle pole...	XM_024447279.1	17.3%	17.1%	616_617delAA;627_3597del
14	human	79848	CSPP1	centrosome and spindle pole...	NM_024790.6	17%	16.8%	1_108del;724_725delAA;735_3663del
15	human	79848	CSPP1	centrosome and spindle pole...	XM_011517607.2	16.9%	16.8%	302_382del;697_698delAA;708_3678del
16	human	79848	CSPP1	centrosome and spindle pole...	XM_024447278.1	16.9%	16.8%	302_382del;697_698delAA;708_3678del
17	human	79848	CSPP1	centrosome and spindle pole...	XM_011517603.2	16.8%	16.6%	302_406del;721_722delAA;732_3702del
18	human	79848	CSPP1	centrosome and spindle pole...	XM_017013850.2	16.8%	16.6%	1_246del;862_863delAA;873_3702del
19	human	79848	CSPP1	centrosome and spindle pole...	NM_001364869.1	16.6%	16.5%	(many diffs)
20	human	79848	CSPP1	centrosome and spindle pole...	XM_017013849.2	16.6%	16.5%	1_246del;862_863delAA;873_3744del
21	human	79848	CSPP1	centrosome and spindle pole...	XM_006716474.3	16.6%	16.4%	(many diffs)
22	human	79848	CSPP1	centrosome and spindle pole...	XM_017013848.2	16.4%	16.3%	(many diffs)
23	human	79848	CSPP1	centrosome and spindle pole...	XM_011517602.2	16.4%	16.2%	1_246del;862_863delAA;873_3801del
24	human	79848	CSPP1	centrosome and spindle pole...	XM_017013847.2	16.3%	16.2%	(many diffs)
25	human	79848	CSPP1	centrosome and spindle pole...	XM_011517601.2	16.2%	16%	1_246del;862_863delAA;873_3843del
26	human	79848	CSPP1	centrosome and spindle pole...	XM_011517600.2	16%	15.9%	(many diffs)
27	human	79848	CSPP1	centrosome and spindle pole...	XM_005251305.4	15.9%	15.8%	(many diffs)
28	human	79848	CSPP1	centrosome and spindle pole...	XM_011517599.2	15.9%	15.7%	(many diffs)
29	human	79848	CSPP1	centrosome and spindle pole...	XM_011517598.2	15.8%	15.6%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

690

ORF length:

624

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61	TTGGCATGGC TGATAATTTG GATGAATTTA TTGAAGAGCA AAAAGCCAGA TTGGCCGAAG
121	ACAAAGCAGA GTTGGAAAGT GATCCACCTT ACATGGAAAT GAAGGGAAAG TTGTCAGCGA
181	AGCTTTCTGA AAACAGTAAG ATACTGATCT CTATGGCTAA GGAAAACATA CCACCAAATA
241	GTCAACAGAC CAGGGGTTCC TTAGGAATTG ATTATGGATT AAGTTTACCA CTTGGAGAAG
301	ACTATGAACG GAAGAAACAT AAATTAAAAG AAGAATTGCG GCAAGATTAC AGACGTTATC
361	TTACTCAGGA AAGGTTGAAA CTTGAACGTA ACAAAGAATA CAATCAGTTT CTCAGGGGTA
421	AGGAAGAATC CAGTGAAAAG TTCAGGCAGG TGGAAAAGAG TACTGAGCCC AAGAGTCAGA
481	GAAATAAAAA ACCTATTGGT CAAGTTAAGC CTGATCTAAC TTCACAAATA CAGACATCTT
541	GTGAAAATTC AGAGGGTCCT AGAAAAGATG TCTTAACTCC TTCAGAGGCA TATGAAGAAC
601	TTCTGAACCA AAGACGACTA GAGGAGGACA GATACCGACA ACTAGATGAT GAAATCGAAT
661	TAAGGAATAG AAGAATTATT AAAAGCAAAT GCCCAACTTT CTTGTACAAA GTtggcatta
721	taagaaagca ttgcttatca atttgttgca acgaac

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