Construct: ORF ccsbBroadEn_13089
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013722.1_s300c1, BRDN0000384943
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLBA1 (122616)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 122616 | CLBA1 | clathrin binding box of aft... | NM_174891.4 | 25.4% | 25.5% | 1_726del;949C>T |
| 2 | human | 122616 | CLBA1 | clathrin binding box of aft... | XM_005267318.4 | 9.6% | 9.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 315
- ORF length:
- 249
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGA AGATTGTGAC CTCAAAGAGC CTGAAGGACT CCTCACTGTC AGCAGCTTCT 121 GTCTCCAGCA TTGCAAAGCC CTGATCCAGA CCAAGCTCTC GGGGCCGCCT GGCAGCAAAC 181 AGGGGAGGCT GATGACATGC AGCCGCTTCC TGAAGACCCC CTCATGCGGA GGTGGCCAGC 241 ACATCACTAT TCCAAGGAAA AGGATGTTCA CTCCACGCAA GCTCAAATTG ACACTCTTTA 301 ATAGCGACGT TTGCTACCCA ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct 361 tatcaatttg ttgcaacgaa c