Construct: ORF ccsbBroadEn_13880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013436.1_s300c1, BRDN0000397015
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IDH3G (3421)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3421 | IDH3G | isocitrate dehydrogenase (N... | NM_004135.4 | 99.9% | 2.6% | 7_8insT |
| 2 | human | 3421 | IDH3G | isocitrate dehydrogenase (N... | NM_174869.3 | 93.8% | 2.7% | (many diffs) |
| 3 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | NM_008323.1 | 87.8% | 2.6% | (many diffs) |
| 4 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_006527843.3 | 86.8% | 2.7% | (many diffs) |
| 5 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_006527844.1 | 76.1% | 3.1% | (many diffs) |
| 6 | mouse | 15929 | Idh3g | isocitrate dehydrogenase 3 ... | XM_017318392.1 | 76.1% | 3.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 213
- ORF length:
- 147
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGC GCTTGAAGGT AGCGACCGTC GCCGGCAGCG CCGCGAAGGC GGTGCTCGGG 121 CCAGCCCTTC TCTGCCGTCC CTGGGAGGTT CTAGGCGCCC ACGAGGTCCC CTCGAGGAAC 181 ATCTTTTCAG AACAAACAAT TCCTCCGTCC GCTAAGTATG GCGGGCGGCA CACGGTGACC 241 ATGATCCCAG GGGATGGCAT CGGGCCAGAG CTCATGCTGC ATGTCAAGTC CGTCTTCAGG 301 CACGCATGTG TACCAGTGGA CTTTGAAGAG GTGCACGTGA GTTCCAATGC TGATGAAGAG 361 GACATTCGCA ATGCCATCAT GGCCATCCGC CGGAACCGCG TGGCCCTGAA GGGCAACATC 421 GAAACCAACC ATAACCTGCC ACCGTCGCAC AAATCTCGAA ACAACATCCT TCGCACCAGC 481 CTGGACCTCT ATGCCAACGT CATCCACTGT AAGAGCCTTC CAGGCGTGGT GACCCGGCAC 541 AAGGACATAG ACATCCTCAT TGTCCGGGAG AACACAGAGG GCGAGTACAG CAGCCTGGAG 601 CATGAGAGTG TGGCGGGAGT GGTGGAGAGC CTGAAGATCA TCACCAAGGC CAAGTCCCTG 661 CGCATTGCCG AGTATGCCTT CAAGCTGGCG CAGGAGAGCG GGCGCAAGAA AGTGACGGCC 721 GTGCACAAGG CCAACATCAT GAAACTGGGC GATGGGCTTT TCCTCCAGTG CTGCAGGGAG 781 GTGGCAGCCC GCTACCCTCA GATCACCTTC GAGAACATGA TTGTGGATAA CACCACCATG 841 CAGCTGGTGT CCCGGCCCCA GCAGTTTGAT GTCATGGTGA TGCCCAATCT CTATGGCAAC 901 ATCGTCAACA ATGTCTGCGC GGGACTGGTC GGGGGCCCAG GCCTTGTGGC TGGGGCCAAC 961 TATGGCCATG TGTACGCGGT GTTTGAAACA GCTACGAGGA ACACCGGCAA GAGTATCGCC 1021 AATAAGAACA TCGCCAACCC CACGGCCACC CTGCTGGCCA GCTGCATGAT GCTGGACCAC 1081 CTCAAGCTGC ACTCCTATGC CACCTCCATC CGTAAGGCTG TCCTGGCATC CATGGACAAT 1141 GAGAATATGC ACACTCCGGA CATCGGGGGC CAGGGCACAA CATCTGAAGC CATCCAGGAC 1201 GTCATCCGCC ACATCCGCGT CATCAACGGC CGGGCCGTGG AGGCCTACCC AACTTTCTTG 1261 TACAAAGTtg gcattataag aaagcattgc ttatcaattt gttgcaacga ac