Construct: ORF ccsbBroadEn_13908

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015685.1_s300c1, BRDN0000455264
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYL5 (4636)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4636 MYL5 myosin light chain 5 NM_001363650.2 99.4% 91% 243G>A;361_362insG
2 human 4636 MYL5 myosin light chain 5 NM_002477.1 75.9% 69.7% 1_123del;366G>A;484_485insG
3 human 4636 MYL5 myosin light chain 5 XM_017008245.1 75.9% 69.7% 1_123del;366G>A;484_485insG
4 human 4636 MYL5 myosin light chain 5 XM_017008246.1 75.9% 69.7% 1_123del;366G>A;484_485insG
5 human 4636 MYL5 myosin light chain 5 XM_006713886.2 40.1% 36.9% 1_588del;831G>A;949_950insG
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
465
ORF length:
399
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA TCAGAACCGA GATGGCTTCA TTGACAAGGA GGACCTGAAG GACACCTATG
121CCTCCCTGGG CAAGACCAAC GTCAAGGACG ACGAGCTGGA CGCCATGCTC AAAGAGGCCT
181CGGGGCCCAT CAACTTCACC ATGTTTCTGA ACCTGTTTGG GGAGAAGCTG AGCGGTACCG
241ACGCCGAGGA GACCATTCTT AACGCCTTCA AGATGCTGGA CCCGGACGGG AAAGGGAAAA
301TCAACAAAGA GTACATCAAG CGTCTGCTGA TGTCCCAGGC TGACAAGATG ACGGCGGAAG
361AGGTGGACCA GATGTTCCAG TTCGCCTCCA TCGATGTGGC GGGCAACCTG GACTACAAGG
421CGCTCAGGCT ACGTGATCAC CCACGGGGAG GAGAAGGAGG AGTGCCCAAC TTTCTTGTAC
481AAAGTTGGca ttataagaaa gcattgctta tcaatttgtt gcaacgaac

Download FASTA (ORF) (Full)

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