Construct: ORF ccsbBroadEn_14084
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004008.1_s300c1, BRDN0000392896
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRT1 (23411)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23411 | SIRT1 | sirtuin 1 | NM_001142498.1 | 81.4% | 81.4% | 0_1ins309 |
| 2 | human | 23411 | SIRT1 | sirtuin 1 | NM_001314049.1 | 79.6% | 77.3% | (many diffs) |
| 3 | human | 23411 | SIRT1 | sirtuin 1 | NM_012238.5 | 74.2% | 74.2% | 1_576del |
| 4 | mouse | 93759 | Sirt1 | sirtuin 1 | NM_001159589.2 | 70.1% | 72.3% | (many diffs) |
| 5 | mouse | 93759 | Sirt1 | sirtuin 1 | NM_019812.3 | 66.5% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1731
- ORF length:
- 1665
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGAT TGGCACAGAT CCTCGAACAA TTCTTAAAGA TTTATTGCCG GAAACAATAC 121 CTCCACCTGA GTTGGATGAT ATGACACTGT GGCAGATTGT TATTAATATC CTTTCAGAAC 181 CACCAAAAAG GAAAAAAAGA AAAGATATTA ATACAATTGA AGATGCTGTG AAATTACTGC 241 AAGAGTGCAA AAAAATTATA GTTCTAACTG GAGCTGGGGT GTCTGTTTCA TGTGGAATAC 301 CTGACTTCAG GTCAAGGGAT GGTATTTATG CTCGCCTTGC TGTAGACTTC CCAGATCTTC 361 CAGATCCTCA AGCGATGTTT GATATTGAAT ATTTCAGAAA AGATCCAAGA CCATTCTTCA 421 AGTTTGCAAA GGAAATATAT CCTGGACAAT TCCAGCCATC TCTCTGTCAC AAATTCATAG 481 CCTTGTCAGA TAAGGAAGGA AAACTACTTC GCAACTATAC CCAGAACATA GACACGCTGG 541 AACAGGTTGC GGGAATCCAA AGGATAATTC AGTGTCATGG TTCCTTTGCA ACAGCATCTT 601 GCCTGATTTG TAAATACAAA GTTGACTGTG AAGCTGTACG AGGAGATATT TTTAATCAGG 661 TAGTTCCTCG ATGTCCTAGG TGCCCAGCTG ATGAACCGCT TGCTATCATG AAACCAGAGA 721 TTGTGTTTTT TGGTGAAAAT TTACCAGAAC AGTTTCATAG AGCCATGAAG TATGACAAAG 781 ATGAAGTTGA CCTCCTCATT GTTATTGGGT CTTCCCTCAA AGTAAGACCA GTAGCACTAA 841 TTCCAAGTTC CATACCCCAT GAAGTGCCTC AGATATTAAT TAATAGAGAA CCTTTGCCTC 901 ATCTGCATTT TGATGTAGAG CTTCTTGGAG ACTGTGATGT CATAATTAAT GAATTGTGTC 961 ATAGGTTAGG TGGTGAATAT GCCAAACTTT GCTGTAACCC TGTAAAGCTT TCAGAAATTA 1021 CTGAAAAACC TCCACGAACA CAAAAAGAAT TGGCTTATTT GTCAGAGTTG CCACCCACAC 1081 CTCTTCATGT TTCAGAAGAC TCAAGTTCAC CAGAAAGAAC TTCACCACCA GATTCTTCAG 1141 TGATTGTCAC ACTTTTAGAC CAAGCAGCTA AGAGTAATGA TGATTTAGAT GTGTCTGAAT 1201 CAAAAGGTTG TATGGAAGAA AAACCACAGG AAGTACAAAC TTCTAGGAAT GTTGAAAGTA 1261 TTGCTGAACA GATGGAAAAT CCGGATTTGA AGAATGTTGG TTCTAGTACT GGGGAGAAAA 1321 ATGAAAGAAC TTCAGTGGCT GGAACAGTGA GAAAATGCTG GCCTAATAGA GTGGCAAAGG 1381 AGCAGATTAG TAGGCGGCTT GATGGTAATC AGTATCTGTT TTTGCCACCA AATCGTTACA 1441 TTTTCCATGG CGCTGAGGTA TATTCAGACT CTGAAGATGA CGTCTTATCC TCTAGTTCTT 1501 GTGGCAGTAA CAGTGATAGT GGGACATGCC AGAGTCCAAG TTTAGAAGAA CCCATGGAGG 1561 ATGAAAGTGA AATTGAAGAA TTCTACAATG GCTTAGAAGA TGAGCCTGAT GTTCCAGAGA 1621 GAGCTGGAGG AGCTGGATTT GGGACTGATG GAGATGATCA AGAGGCAATT AATGAAGCTA 1681 TATCTGTGAA ACAGGAAGTA ACAGACATGA ACTATCCATC AAACAAATCA TACCCAACTT 1741 TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac