Construct: ORF ccsbBroadEn_14113

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003060.1_s300c1, BRDN0000386681
DNA Barcode:
None
Epitope Tag:
None
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
TPK1 (27010)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350885.1 99% 4.3% 14_16delAGAinsG;25A>G
2 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350886.1 99% 4.3% 14_16delAGAinsG;25A>G
3 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350887.1 99% 4.3% 14_16delAGAinsG;25A>G
4 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350889.1 99% 4.3% 14_16delAGAinsG;25A>G
5 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350893.1 99% 4.3% 14_16delAGAinsG;25A>G
6 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350894.1 99% 4.3% 14_16delAGAinsG;25A>G
7 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516047.2 99% 4.3% 14_16delAGAinsG;25A>G
8 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516048.1 99% 4.3% 14_16delAGAinsG;25A>G
9 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011982.1 99% 4.3% 14_16delAGAinsG;25A>G
10 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446717.1 99% 4.3% 14_16delAGAinsG;25A>G
11 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350895.1 92.1% 3.1% 0_1ins31;2T>A
12 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_024446716.1 92.1% 3.1% 0_1ins31;2T>A
13 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011980.2 83.2% 3.6% 1_78del;92_94delAGAinsG;103A>G
14 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011981.2 83.2% 3.6% 1_78del;92_94delAGAinsG;103A>G
15 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350882.1 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
16 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350883.1 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
17 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350884.2 57% 2.5% 1_303del;317_319delAGAinsG;328A>G
18 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350879.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
19 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_022445.4 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
20 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_005249970.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
21 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516039.2 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
22 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011974.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
23 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011975.1 55.8% 2.4% 1_318del;332_334delAGAinsG;343A>G
24 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001042482.1 55.5% 3.6% (many diffs)
25 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350880.1 55.5% 3.6% (many diffs)
26 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516037.2 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
27 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011970.1 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
28 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011971.1 51.3% 2.2% 1_381del;395_397delAGAinsG;406A>G
29 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516031.1 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
30 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516032.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
31 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516033.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
32 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516034.2 50.4% 2.2% 1_396del;410_412delAGAinsG;421A>G
33 human 27010 TPK1 thiamin pyrophosphokinase 1 NM_001350881.1 47.1% 2.4% (many diffs)
34 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516035.3 42.7% 2.2% (many diffs)
35 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011969.1 42.5% 2.2% (many diffs)
36 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_017011972.1 40.4% 2.4% (many diffs)
37 human 27010 TPK1 thiamin pyrophosphokinase 1 XM_011516040.2 39.6% 2.4% (many diffs)
38 human 27010 TPK1 thiamin pyrophosphokinase 1 NR_146936.2 14.5% (many diffs)
39 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506219.3 53.5% 1.8% (many diffs)
40 mouse 29807 Tpk1 thiamine pyrophosphokinase NM_013861.4 48.2% 1.6% (many diffs)
41 mouse 29807 Tpk1 thiamine pyrophosphokinase XM_006506218.3 32% .8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
102
ORF length:
36
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT CCAAAAGAGT AGAAGAAGAA GACTTAAAGG TTGATGTGAT CGTGACACTG
121GGAGGCCTTG CTGGGCGTTT TGACCAGATT ATGGCATCTG TGAATACCTT GTTCCAAGCG
181ACTCACATCA CTCCTTTTCC AATTATAATA ATCCAAGAGG AATCGCTGAT CTACCTGCTC
241CAACCAGGAA AGCACAGGTT GCATGTAGAC ACTGGAATGG AGGGTGATTG GTGTGGCCTT
301ATTCCTGTTG GACAGCCTTG TATGCAGGTT ACAACCACAG GCCTCAAGTG GAACCTCACA
361AATGATGTGC TTGCTTTTGG AACATTGGTC AGTACTTCCA ATACCTACGA CGGGTCTGGT
421GTTGTGACTG TGGAAACTGA CCACCCACTC CTCTGGACCA TGGCCATCAA AAGCTACCCA
481ACTTTCTTGT ACAAAGTtgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
541c

Download FASTA (ORF) (Full)