Construct: ORF ccsbBroadEn_14548

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019054.1_s300c1, BRDN0000458672
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BLK (640)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 640 BLK BLK proto-oncogene, Src fam... NM_001715.3 100% 100%
2 human 640 BLK BLK proto-oncogene, Src fam... XM_011543824.1 95.1% 95.1% 952_1029del
3 human 640 BLK BLK proto-oncogene, Src fam... XM_011543825.3 95.1% 95.1% 952_1029del
4 human 640 BLK BLK proto-oncogene, Src fam... NM_001330465.2 85.9% 85.9% 0_1ins213
5 human 640 BLK BLK proto-oncogene, Src fam... XM_011543827.1 81.7% 81.7% 0_1ins213;739_816del
6 human 640 BLK BLK proto-oncogene, Src fam... XM_011543828.3 62.7% 51.1% (many diffs)
7 human 640 BLK BLK proto-oncogene, Src fam... XM_011543829.3 62.5% 51.2% (many diffs)
8 mouse 12143 Blk B lymphoid kinase NM_007549.2 83.8% 86.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1584
ORF length:
1515
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat ggggctggta agtagcaaaa agccggacaa ggaaaagccg atcaaagaga
121aggacaaggg ccaatggagc cccctgaagg tcagcgccca agacaaggac gccccgccac
181tgccgcccct ggttgtcttc aaccacctta ctcctccacc gcccgatgaa cacctggatg
241aagacaagca tttcgtggtg gctctgtatg actacaccgc tatgaatgat cgggacctgc
301agatgctgaa gggggagaag ctacaggtcc tgaagggaac tggagactgg tggctggcca
361ggtcactcgt cacaggaaga gaaggctatg tgcccagtaa ctttgtggcc cgagtggaga
421gcctggaaat ggaaaggtgg ttctttagat cacagggtcg gaaggaggct gagaggcagc
481ttcttgctcc aatcaacaag gccggctcct ttcttatcag agagagtgaa accaacaaag
541gtgccttctc cctgtctgtg aaggatgtca ccacccaggg ggagctgatc aagcactata
601agatccgctg cctggatgaa gggggctact acatctcccc ccggatcacc ttcccctcgc
661tccaggccct ggtgcagcac tattctaaga agggggatgg tctatgccag aggctgaccc
721tgccctgtgt gcgcccggcc ccgcagaatc cctgggccca ggatgaatgg gagatccccc
781ggcagtctct caggctggtc aggaaactcg ggtctggaca attcggcgaa gtctggatgg
841gttactacaa aaacaacatg aaggtggcca ttaagacgct gaaggaggga accatgtctc
901cagaagcctt tctgggtgag gccaacgtga tgaaggctct gcagcacgag cggctggtcc
961gactctacgc agtggtcacc aaggagccca tctacattgt caccgagtac atggccagag
1021gatgcctgct ggatttcctg aagacagatg aagggagcag attgtcactc ccaaggctga
1081ttgacatgtc ggcgcagatt gctgaaggga tggcatacat tgagcgcatg aattccatcc
1141accgcgacct gcgggcggcc aacatcctgg tgtctgaggc cttgtgctgc aaaattgctg
1201attttggctt ggctcgaatc atcgacagtg aatacacggc ccaagagggg gccaagttcc
1261ccatcaagtg gacagccccg gaagccatcc acttcggggt cttcaccatc aaagcagacg
1321tgtggtcgtt tggagtcctc ctgatggaag ttgtcactta tgggcgggtg ccatacccag
1381ggatgagcaa ccccgaggtc atccgcaacc tggagcgcgg ctaccgcatg ccgcgccccg
1441acacctgccc gcccgagctg taccgcggcg tcatcgccga gtgctggcgc agccggcccg
1501aggagcggcc caccttcgag ttcctgcagt cggtgctgga ggacttctac acggccaccg
1561agcggcagta cgagctgcag cccttgccaa ctttcttgta caaagttggc attataagaa
1621agcattgctt atcaatttgt tgcaacgaac

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