Construct: ORF ccsbBroadEn_14613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018817.1_s300c1, BRDN0000458113
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCK (1633)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1633 | DCK | deoxycytidine kinase | NM_000788.3 | 99.7% | 99.6% | 29_30delCGinsNN |
| 2 | mouse | 13178 | Dck | deoxycytidine kinase | NM_007832.4 | 88.2% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 879
- ORF length:
- 780
- Sequence:
-
1 TATGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCCACCCCG CCCAAGAGAA 121 GCTGCCNNTC TTTCTCAGCC AGCTCTGAGG GGACCCGCAT CAAGAAAATC TCCATCGAAG 181 GGAACATCGC TGCAGGGAAG TCAACATTTG TGAATATCCT TAAACAATTG TGTGAAGATT 241 GGGAAGTGGT TCCTGAACCT GTTGCCAGAT GGTGCAATGT TCAAAGTACT CAAGATGAAT 301 TTGAGGAACT TACAATGTCT CAGAAAAATG GTGGGAATGT TCTTCAGATG ATGTATGAGA 361 AACCTGAACG ATGGTCTTTT ACCTTCCAAA CATATGCCTG TCTCAGTCGA ATAAGAGCTC 421 AGCTTGCCTC TCTGAATGGC AAGCTCAAAG ATGCAGAGAA ACCTGTATTA TTTTTTGAAC 481 GATCTGTGTA TAGTGACAGG TATATTTTTG CATCTAATTT GTATGAATCT GAATGCATGA 541 ATGAGACAGA GTGGACAATT TATCAAGACT GGCATGACTG GATGAATAAC CAATTTGGCC 601 AAAGCCTTGA ATTGGATGGA ATCATTTATC TTCAAGCCAC TCCAGAGACA TGCTTACATA 661 GAATATATTT ACGGGGAAGA AATGAAGAGC AAGGCATTCC TCTTGAATAT TTAGAGAAGC 721 TTCATTATAA ACATGAAAGC TGGCTCCTGC ATAGGACACT GAAAACCAAC TTCGATTATC 781 TTCAAGAGGT GCCTATCTTA ACACTGGATG TTAATGAAGA CTTTAAAGAC AAATATGAAA 841 GTCTGGTTGA AAAGGTCAAA GAGTTTTTGA GTACTTTGTT GCCAACTTTC TTGTACAAAG 901 TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC 961 AAAATAAAAT CATTATTGCC ATCCAGCTGA TTCCCGA