Construct: ORF ccsbBroadEn_14742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018850.1_s300c1, BRDN0000458146
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PFKFB1 (5207)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | NM_001271805.1 | 67.1% | 48.6% | (many diffs) |
| 2 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | NM_002625.4 | 61.8% | 42.5% | (many diffs) |
| 3 | human | 5207 | PFKFB1 | 6-phosphofructo-2-kinase/fr... | XM_017029577.1 | 57.6% | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 480
- ORF end:
- 1413
- ORF length:
- 933
- Sequence:
-
1 TACGTCAGCC AGCCTATGGT CCGTTGCAAT GAATTAGAGC TATCCACGAA TTTACACGCA 61 GAGACTTAAT CAGAAAACTT CGATAGCATT GGCGTCACGT AATAGGTATA GCATCTGAAG 121 TTGACTGCCT AAATTACCGA GGCAGCAGGC CAGTCAACGC ATACCATCCT GATAGGTAAA 181 TGCCTAAAGC CGCTACCCGA GATGACTATC ATCAATTCAC ATCTTTTGAT TGAGCAGAGT 241 CGCCCAGATT ACAAGGGACC ATTTGTAAAT GTTGGGTATT AACTTTGTTA TGAACCAAGC 301 ATTTTGTTTT TGGTTTTTTC AGGAAACATC TCAGTCACAT GTGATGCGAC ACACTATCCA 361 GTATTTCGGC TGGACCCCAA ATAATGATTT TATTTTGACT GATAGTGACC TGTTCGTTGC 421 AACAAATTGA TGAGCAATGC TTTTTTATAA TGCCAACTTT GTACAAAAAA GTTGGCACCA 481 TGAAGCTCTG CGTGACTGTC CTGTCTCTCC TCATGCTAGT AGCTGCCTTC TGCTCTCCAG 541 CGCTCTCAGC ACCAAGTAAG TCTACTTTTG CAGCTGCTAT TTCGAGTCAA GGTGTAGGCA 601 GAGTCCTTTT TTCTAGTCAT GGCTGGCAAA CAGTGGGATC TGGGGATGGG ACAAAAGGCA 661 GCTTGCCAAC TTTCTTGTAC AAAGTTGGCA TTATCAGAAA GCATTGCTTA TCACTTCGCT 721 GCAACGAACA GGTCACCATC AGTCAAAATA AAATCATTAT TGCCATCCAG CTGATTCCCG 781 ACataattgc agaaaacatc aggcaagtga aacttggcag ccctgattat atagactgtg 841 accgggaaaa ggttctggaa gactttctaa agagaattga gtgctatgag gtcaactacc 901 aacccttgga tgaggaactg gacagccacc tgtcctacat caagatcttc gacgtgggca 961 cacgctacat ggtgaaccga gtgcaggatc acatccagag ccgcacagtc tactacctca 1021 tgaatatcca tgtcacacct cgctccatct acctttgccg acatggcgag agtgaactca 1081 acatcagagg ccgcatcgga ggtgactctg gcctctcagt tcgcggcaag cagtatgcct 1141 atgccctggc caacttcatt cagtcccagg gcatcagctc cctgaaggtg tggaccagtc 1201 acatgaagag gaccatccag acagctgagg ccctgggtgt cccctatgag cagtggaagg 1261 ccctgaatga gattgatgcg ggtgtctgtg aggagatgac ctatgaagaa atccaggaac 1321 attaccctga agaatttgca ctgcgagacc aagataaata tcgctaccgc tatcccaagg 1381 gagagtccta tTATGATTTT ATTTTGACTC GATAGTCGAC CTATTCGTTG CAAGCAAATT 1441 CGATGAGCAA TGCTTTTTTA TAATGCCAAC TTTGTACAAA AAAGTTGGCA CCATGAAGCT 1501 CTGCGTGACT GTCCTGTCTC TCCTCATGCT AGTAGCTGCC TTCTGCTCTC CAGCGCTCTC 1561 AGCACCAAGT AAGTCTACTT TTGCAGCTGC TATTTCGAGT CAAGGTGTAG GCAGAGTCCT 1621 TTTTTCTAGT CATGGCTGGC AAACAGTGGG ATCTGGGGAT GGGACAAAAG GCAGCTTGCC 1681 AACTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 1741 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGGGGT CCGAGTGATA GCCCGGATGT 1801 CGTGTAGCAT TACATGGTCA TAACTGTTTC CTGGCATCTC TGGCCCGTGT CTCATATCTC 1861 AGGTGTTACA GCTTCCAGAG TTTTTCCGAA ACCTTTAACC CATTCTCCCC ATTTGCTATT 1921 AGGATCAAAA AAAAAACATT CCGCATCTTT CTAAGGAGAC CACATATTAA CAGACAACAA 1981 AGAAACGTGC CTTACCATAC GGATAAAGAA CACCCTCCCT CGACAGCCCA AATACAAAAT 2041 GACAAAATCT AAATATTCAA TATACTCTCG GAGAAGACAT AAATCGCAAT ATAAGTTTTT 2101 TTGTTATTTT ATAAACCAAC CCCCAATGAG GATGTGCAAA TGTACACCAT ATAAAAATTA 2161 TATTATTAGA TAATTTTGAT TAAAGCCTTC CTCGCCTGAA TCAGTTCTGT AGGGTCATGC 2221 CAAATTAATC ATGGACGTAC CCAATAGACG GCATAAGCCT AGCCGAATCC CTGAATTCCA 2281 TAGCCGGGGA GCATGGAATT TGGG