Construct: ORF ccsbBroadEn_14759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018902.1_s300c1, BRDN0000458198
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PIK3CA (5290)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.4 | 99.3% | 21.2% | (many diffs) |
| 2 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_006713658.4 | 99.3% | 21.2% | (many diffs) |
| 3 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | XM_011512894.2 | 99.3% | 21.2% | (many diffs) |
| 4 | human | 5290 | PIK3CA | phosphatidylinositol-4,5-bi... | NM_006218.2:c.3204_3205insA | 99.2% | 21.1% | (many diffs) |
| 5 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | NM_008839.2 | 89.1% | 20.8% | (many diffs) |
| 6 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535409.3 | 89.1% | 20.8% | (many diffs) |
| 7 | mouse | 18706 | Pik3ca | phosphatidylinositol-4,5-bi... | XM_006535410.3 | 89.1% | 20.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 802
- ORF length:
- 705
- Sequence:
-
1 ACTGCTTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGC CTCCACGACC ATCATCAGGT 121 GAACTGTGGG GCATCCACTT GATGCCCCCA AGAATCCTAG TAGAATGTTT ACTACCAAAT 181 GGAATGATAG TGACTTTAGA ATGCCTCCGT GAGGCTACAT TAATAACCAT AAAGCATGAA 241 CTATTTAAAG AAGCAAGAAA ATACCCCCTC CATCAACTTC TTCAAGATGA ATCTTCTTAC 301 ATTTTCGTAA GTGTTACTCA AGAAGCAGAA AGGGAAGAAT TTTTTGATGA AACAAGACGA 361 CTTTGTGACC TTCGGCTTTT TCAACCCTTT TTAAAAGTAA TTGAACCAGT AGGCAACCGT 421 GAAGAAAAGA TCCTCAATCG AGAAATTGGT TTTGCTATCG GCATGCCAGT GTGTGAATTT 481 GATATGGTTA AAGATCCAGA AGTACAGGAC TTCCGAAGAA ATATTCTGAA CGTTTGTAAA 541 GAAGCTGTGG ATCTTAGGGA CCTCAATTCA CCTCATAGTA GAGCAATGTA TGTCTATCCT 601 CCAAATGTAG AATCTTCACC AGAATTGCCA AAGCACATAT ATAATAAATT AGATAAAGGG 661 CAAATAATAG TGGTGATCTG GGTAATAGTT TCTCCAAATA ATGACAAGCA GAAGTATACT 721 CTGAAAATCA ACCATGACTG TGTACCAGAA CAAGTAATTG CTGAAGCAAT CAGAAAAAAC 781 TCGAAGTATG TTGCTATCCT CTGACACTAA ACTCTGTGTT TTAGATATCA GCAAGTATAT 841 TTTAAAGTGT GTGGATGTGA TGAATACTTC CTAGAAAAAT ATCCTCTGAG TCAGTATAAG 901 TATataagaa gctgtataat gcttgggagg atgcccaatt tgatgttgat ggctaaagaa 961 agcctttatt ctcaactgcc aatggactgt tttacaatgc catcttattc cagacgcatt 1021 tccacagcta caccatatat gaatggagaa acatctacaa aatccctttg ggttataaat 1081 agtgcactca gaataaaaat tctttgtgca acctacgtga atgtaaatat tcgagacatt 1141 gataagatct atgttcgaac aggtatctac catggaggag aacccttatg tgacaatgtg 1201 aacactcaaa gagtaccttg ttccaatccc aggtggaatg aatggctgaa ttatgatata 1261 tacattcctg atcttcctcg tgctgctcga ctttgccttt ccatttgctc tgttaaaggc 1321 cgaaagggtg ctaaagagga acactgtcca ttggcatggg gaaatataaa cttgtttgat 1381 tacacagaca ctctagtatc tggaaaaatg gctttgaatc tttggccagt acctcatgga 1441 ttagaagatt tgctgaaccc tattggtgtt actggatcaa atccaaataa agaaactcca 1501 tgcttagagt tggagtttga ctggttcagc agtgtggtaa agttcccaga tatgtcagtg 1561 attgaagagc atgccaattg gtctgtatcc cgagaagcag gatttagcta ttcccacgca 1621 ggactgagta acagactagc tagagacaat gaattaaggg aaaatgacaa agaacagctc 1681 aaagcaattt ctacacgaga tcctctctct gaaatcactg agcaggagaa agattttcta 1741 tggagtcaca gacactattg tgtaactatc cccgaaattc tacccaaatt gcttctgtct 1801 gttaaatgga attctagaga tgaagtagcc cagatgtatt gcttggtaaa agattggcct 1861 ccaatcaaac ctgaacaggc tatggaactt ctggactgta attacccaga tcctatggtt 1921 cgaggttttg ctgttcggtg cttggaaaaa tatttaacag atgacaaact ttctcagtat 1981 ttaattcagc tagtacaggt cctaaaatat gaacaatatt tggataactt gcttgtgaga 2041 tttttactga agaaagcatt gactaatcaa aggattgggc actttttctt ttggcattta 2101 aaatctgaga tgcacaataa aacagttagc cagaggtttg gcctgctttt ggagtcctat 2161 tgtcgtgcat gtgggatgta tttgaagcac ctgaataggc aagtcgaggc aatggaaaag 2221 ctcattaact taactgacat tctcaaacag gagaagaagg atgaaacaca aaaggtacag 2281 atgaagtttt tagttgagca aatgaggcga ccagatttca tggatgctct acagggcttt 2341 ctgtctcctc taaaccctgc tcatcaacta ggaaacctca ggcttgaaga gtgtcgaatt 2401 atgtcctctg caaaaaggcc actgtggttg aattgggaga acccagacat catgtcagag 2461 ttactgtttc agaacaatga gatcCTCTTT AAAAATGGGG ATGATTTACG CCAAGATATG 2521 CTAACACTTC CAANTTATTC GTATATGGGA AAATATCTGC AAAAATCAAG GTCNTGATCT 2581 TCGAATGTTA CCTTATGGTT GTCTGTCAAT CGGTGACTGT GTGGGACTTA TGAGGTGGGT 2641 GCGAAATTCT CACACTATTA TGCAAATTCA GTGCAAAGGC GGCTTGAAAG GTGCACTGCA 2701 GTTCAACAGC CACACACTAC ATCAGTGGCT CAAAGACAAG AACAAAGGAG AAATATATGA 2761 TGCAGCCATT GACCTGTTTA CACGTTCATG TGCTGGATAC TGTGTAGCTA CCTTCATTTT 2821 GGGAATTGGA GATCGTCACA ATAGTAACAT CATGGTGAAA GACGATGGAC AACTGTTTCA 2881 TATAGATTTT GGACACTTTT TGGATCACAA GAAGAAAAAA TTTGGTTATA AACGAGAACG 2941 TGTGCCATTT GTTTTGACAC AGGATTTCTT AATAGTGATT AGTAAAGGAG CCCAAGAATG 3001 CACAAAGACA AGAGAATTTG AGAGGTTTCA GGAGATGTGT TACAAGGCTT ATCTAGCTAT 3061 TCGACAGCAT GCCAATCTCT TCATAAATCT TTTCTCAATG ATGCTTGGCT CTGGAATGCC 3121 AGAACTACAA TCTTTTGATG ACATTGCATA CATTCGAAAG ACCCTAGCCT TAGATAAAAC 3181 TGAGCAAGAG GTTTTGGAGT ATTTCATGAA ACAAATGAAT GATGCACATC ATGGTGGCTG 3241 GACAACAAAA ATGGATTGGA TCTTCCACAC AATTAAACAG CATGCATTGA ACTTGCCAAC 3301 TTTCTTGTAC AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA 3361 GGTCACTATC AGTCAAAATA AAATCATTAT TGCCATCCAG GCTGAATCCC CCCC