Construct: ORF ccsbBroadEn_15138
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018940.1_s300c1, BRDN0000458236
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PINK1 (65018)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 65018 | PINK1 | PTEN induced kinase 1 | NM_032409.3 | 96.8% | 32.2% | (many diffs) |
2 | human | 102464833 | MIR6084 | microRNA 6084 | NR_106732.1 | 6.5% | 0_1ins82;110_111ins1500 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 934
- ORF length:
- 837
- Sequence:
-
1 ACCGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG CGGTGCGACA GGCGCTGGGC 121 CGCGGCCTGC AGCTGGGTCG AGCGCTGCTG CTGCGCTTCA CGGGCTGTGT CCGCGGGGAG 181 CGTCCAGGCT GGGCCGCAGG ACCGGGCGCG GAGCCTCGCA GGGTCGGGCT CGGGCTCCCT 241 AACCGTCTCC GCTTCTTCCG CCAGTCGGTG GCCGGGCTGG CGGCGCGGTT GCAGCGGCAG 301 TTCGTGGTGC GGGCCTGGGG CTGCGCGGGC CCTTGCGGCC GGGCAGTCTT TCTGGCCTTC 361 GGGCTAGGGC TGGGCCTCAT CGAGGAAAAA CAGGCGGAGA GCCGGCGGGC GGTCTCGGCC 421 TGTCAGGAGA TCCAGGCAAT TTTTACCCAG AAAAGCAAGC CGGGGCCTGA CCCGTTGGAC 481 ACGAGACGCT TGCAGGGCTT TCGGCTGGAG GAGTATCTGA TAGGGCAGTC CATTGGTAAG 541 GGCTGCAGTG CTGCTGTGTA TGAAGCCACC ATGCCTACAT TGCCCCAGAA CCTGGAGGTG 601 ACAAAGAGCA CCGGGTTGCT TCCAGGGAGA GGCCCAGTAC CAGTGCACCA GAGAAGGGCA 661 GGAGCGAGCT CCGGGGGCCC CTGCCTTCCC CTTGGCCATC AAGATGATGT GGAACATCTC 721 GGCAGGTTCC TCCAGCGAAG CCATCTTGAA CACAATGAGC CAGGAGCTGG TCCCAGCGAG 781 CCGAGTGGCC TTGGCTTGAG Ggtatggagc agtcacttac agaaaatcca agagaggtcc 841 caagcaacta gcccctcacc ccaacatcat ccgggttctc cgcgccttca cctcttccgt 901 gccgctgctg ccaggggccc tggtcgacta ccctgatgtg ctgccctcac gcctccaccc 961 tgaaggcctg ggccatggcc ggacgctgtt cctcgttatg aagaactatc cctgtaccct 1021 gcgccagtac ctttgtgtga acacacccag cccccgcctc gGCGCCATGA TGCTGCTGCA 1081 GCTGCTGGAA GGCGTGGACC ATCTGGTTCA ACAGGGCATC GCGCACAGAG ACTGAAATCC 1141 GACAACATCC TTGTGGAGCT GGACCCAGAC GGCTGCCCCT GGCTGGTGAT CGCAGATTTT 1201 GGCTGCTGCC TGGCTGATGA GAGCATCGGC CTGCAGTTGC CCTTCAGCAG CTGGTACGTG 1261 GATCGGGGCG GAAACGGCTG TCTGATGGCC CCAGAGGTGT CCACGGCCCG TCCTGGCCCC 1321 AGGGCAGTGA TTGACTACAG CAAGGCTGAT GCCTGGGCAG TGGGAGCCAT CGCCTATGAA 1381 ATCTTCGGGC TTGTCAATCC CTTCTACGGC CAGGGCAAGG CCCACCTTGA AAGCCGCAGC 1441 TACCAAGAGG CTCAGCTACC TGCACTGCCC GAGTCAGTGC CTCCAGACGT GAGACAGTTG 1501 GTGAGGGCAC TGCTCCAGCG AGAGGCCAGC AAGAGACCAT CTGCCCGAGT AGCCGCAAAT 1561 GTGCTTCATC TAAGCCTCTG GGGTGAACAT ATTCTAGCCC TGAAGAATCT GAAGTTAGAC 1621 AAGATGGTTG GCTGGCTCCT CCAACAATCG GCCGCCACTT TGTTGGCCAA CAGGCTCACA 1681 GAGAAGTGTT GTGTGGAAAC AAAAATGAAG ATGCTCTTTC TGGCTAACCT GGAGTGTGAA 1741 ACGCTCTGCC AGGCAGCCCT CCTCCTCTGC TCATGGAGGG CAGCCCTGTT GCCAACTTTC 1801 TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC 1861 ACTATCAGTC AAAATAAAAT CATTATTGCC ATCCAGCCGT T