Construct: ORF ccsbBroadEn_15148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018820.1_s300c1, BRDN0000458116
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PANK3 (79646)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79646 | PANK3 | pantothenate kinase 3 | NM_024594.4 | 99.1% | 54.9% | (many diffs) |
| 2 | mouse | 211347 | Pank3 | pantothenate kinase 3 | NM_145962.2 | 92.6% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 785
- ORF length:
- 687
- Sequence:
-
1 TAGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG AAGATCAAAG ATGCCAAGAA 121 ACCCTCTTTC CCATGGTTTG GCATGGACAT TGGGGGAACT CTAGTAAAGC TCTCGTACTT 181 TGAACCTATT GATATCACAG CAGAGGAAGA GCAAGAAGAA GTTGAGAGTT TAAAAAGTAT 241 TCGGAAATAT TTGACTTCTA ACGTGGCATA TGGATCCACC GGCATTCGGG ATGTACACCT 301 TGAACTGAAA GATTTAACAC TTTTTGGCCG AAGAGGGAAC TTGCACTTTA TCAGGTTTCC 361 AACCCAGGAC CTGCCTACTT TTATCCAAAT GGGAAGAGAT AAAAACTTCT CAACAATTGC 421 AGAACGGTGC TATGTGCTAC AGGAGGGTGG TGCTTACAAG TTTGAAAAAG ATTTTCGCAC 481 AATTGGAAAC CTCCACCTGC ACAAACTGGA TGAACTTGAC TGCCTTGTAA AGGGCTTGCT 541 GTATATAGAC TCTGTCAGTT TCAATGGACA AGCCGAGTGC TATTATTTTG CTAATGCCTC 601 AGAACNTGAG CGATGCCAAA AGATGCCTTT TAACCTGGAT GATCCCTATC CACTGCTTGT 661 AGTGAACATT GGCTCAGGAG TCAGTATTTT AGCAGTCCAT TCCAAAGACA ACTATAAACG 721 AGTGACTGGG ACANGCCTTT GGAGGGGGTA CCTTTCTGGG TTTATGCAGT TTATTGACTG 781 GCTGTGAAAG TTTTGAAGAG GCTCTTGAAA TGGCATCCAA AGGTGATAGC ACACAAGCTG 841 ACAAGCTGGT CCGTGATATT TATGGAGGAG ATTATGAAAG ATTTGGGTTT GCCAAGGTTG 901 GGCTTGTAGC ATCTAGTTTT GGGAATATGA TTTATAAGGA GAAGCGAGAA TCTGTTAGTA 961 AAGAAGATCT GGCAAGAGCT ACTTTAGTTA CTATCACCAA TAACATTGGT TCTGTGGCAC 1021 GAATGTGTGC TGTTAATGAG AAAATAAACA GAGTTGTCTT TGTTGGAAAC TTTTTACGTG 1081 TCAATACCCT CTCAATGAAA CTTTTGGCAT ATGCACTGGA TTACTGGTCA AAAGGTCAAC 1141 TAAAAGCATT GTTTCTAGAA CATGAGGGTT ACTTTGGAGC AGTTGGTGCA CTTCTTGGGC 1201 TGCCAAATTT CAGCTTGCCA ACTTTCTTGT ACAAAGTTGG CATTATAAGA AAGCATTGCT 1261 TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTGCCATCC 1321 AGCTCCTCAA GA