Construct: ORF ccsbBroadEn_15235
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018803.1_s300c1, BRDN0000458099
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RSKR (124923)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124923 | RSKR | ribosomal protein S6 kinase... | NM_001174103.2 | 66% | 7.1% | (many diffs) |
| 2 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532965.3 | 55.8% | 8.6% | (many diffs) |
| 3 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | NM_001287206.1 | 55.5% | 8.6% | (many diffs) |
| 4 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388406.3 | 55.1% | (many diffs) | |
| 5 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532966.3 | 50.8% | 9.2% | (many diffs) |
| 6 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388407.3 | 48.4% | (many diffs) | |
| 7 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XM_006532967.3 | 44.6% | 9.2% | (many diffs) |
| 8 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_388408.3 | 20.4% | (many diffs) | |
| 9 | mouse | 216976 | BC030499 | cDNA sequence BC030499 | XR_001779956.1 | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 392
- ORF length:
- 294
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG GGAGCAGTAA GCTGTCGGCA 121 GGGGCAGCAC ACCCAGCAGG GGGAACACCA CCCGGGTGGC TGTCCCTCAC AAGCAGGGTG 181 GCAACATCCG GGGTCCCTGG GCCCGAGGCT GGAAGAGCCT CTGGACAGGT TTGGGAACCA 241 TCAGGTCAGA TCTGGAAGAA CTCTGGGAAC TACGGGGGCA CCACTATCTG CACCAGGAAT 301 CCCTAAAGCC AGCCCCAGTA CTGGTAGAGA AGCCTCTGCC AGAGTGGCCA GTGCCTCAGT 361 TCATCAACCT CTTTCTACCA GAGTTTCCCA TTAGGCCCAT TAGGGGGCAG CAGCAGCTGA 421 AGATTTTAGG CCTCGTGGCT AAAGGCTCCT TTGGAACTGT CCTCAAGGTG CTAGATTGCA 481 CCCAGAAAGC TGTATTTGCA GTGAAGGTGG TGCCCAAGGT AAAGGTCCTA CAGAGGGATA 541 CCGTGAGGCA GTGCAAAGAG GAGGTTAGCA TCCAGCGACA GATCAACCAT CCCTTTGTAC 601 ACAGCTTGGG GGACAGCTGG CAGGGAAAAC GGCACCTTTT CATTATGTGT AGCTACTGCA 661 GCACAGATCT GTACTCCCTT TGGTCGGCTG TTGGCTGCTT TCCTGAGGCT TCCATCCGTC 721 TCTTTGCTGC CGAGTTGGTG CTGGTACTGT GTTATCTCCA TGACTTGGGC ATCATGCATC 781 GAGATGTGAA GATGGAGAAT ATTCTTCTAG ATGAACGAGG CCATCTGAAA CTGACAGACT 841 TTGGTCTGTC CCGCCACGTG CCCCAGGGAG CTCAAGCCTA CACTATCTGT GGCACTTCTT 901 CAGTACATGG GTGAGAGAGG TTTGCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG 961 CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT 1021 GCCATCCAGC TGATTC