Construct: ORF ccsbBroadEn_15332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018786.1_s300c1, BRDN0000458082
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLK3 (1198)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1198 | CLK3 | CDC like kinase 3 | NM_003992.4 | 11.3% | 11.2% | 1_1302del;1456C>T |
| 2 | human | 1198 | CLK3 | CDC like kinase 3 | NM_001130028.1 | 8.7% | 8.6% | 1_1746del;1900C>T |
| 3 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313061.1 | 19% | 20% | (many diffs) |
| 4 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_006510739.3 | 10.4% | 11% | (many diffs) |
| 5 | mouse | 102414 | Clk3 | CDC-like kinase 3 | XM_017313060.1 | 10.4% | 11% | (many diffs) |
| 6 | mouse | 102414 | Clk3 | CDC-like kinase 3 | NM_007713.4 | 8% | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 381
- ORF end:
- 549
- ORF length:
- 168
- Sequence:
-
1 NNANGNNTTN NNTTNGNNTG ANNNTNANNT CATCCTCTCA AAAACTCATT ACCACCAGAT 61 GGTACTGTAT TTTTAAACCT TTCAAATGAA AATAGTTTCC ACCAATGAAC ATAAAGAAAA 121 AGGCAGGATA GTTTTTTTAG TTTACACAAT GAAAAAAGCC CAGAAACGAC AAAGAAGAAA 181 CGGAACAAAC CCCAGACCCA AAAAACAAGA CCCCCCCCCC CCAGGAAACA GCCCAGACCA 241 TGTAATACAA CACACTGGCC AGTATTTCAG CTGGACCGCA AATAATGATT TTATTTTGAC 301 TGATAGTGAC CTGTTCGTTG CAACAAATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT 361 TGTACAAAAA AGTTGGCACC ATGCTCCAAG ACTCCCTGGA GCACGTGCAG CTGTTTGACC 421 TGATGAGGAG GATGTTAGAA TTTGACCCTG CCCAGCGCAT CACACTGGCC GAGGCCCTGC 481 TGCACCCCTT CTTTGCTGGC CTGACCCCTG AGGAGCGGTC CTTCCACACC AGCTGCAACC 541 CAAGCAGATT GCTAACTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA 601 TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAN NNTNNTNTNN NNCNNNNGNT 661 NNNNNNCGGA CGTCGAGTCG CATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG 721 TGTCTCAAAA TCTCTACCCA TAAAAAAAAA AGGCGGGGGG GGGGCCTTTA CCCCCCTATC 781 TAGCAGGATT GAAAAAACTA TCCTCAGAAG CCAGAAAAAC AAAAAACACT CTGAATAGGA 841 GAAGCTACGG GTTCCTGCTC CTCTAGAGAC TGCCCCAACA GAAGTAAACC CTGAGACAAC 901 CCGTATAACA AACGATGATT CACAATCGAC GAAAGAAGTA CCGCCTGTGA CCGAAAAGAA 961 CAAGCACACA CACACTGCAA CACTGCTGAA CTGTGACACG ATACCGATGA CTGGTATCGA 1021 GACATGACGT TCCATTGTCA TGCACCGTTG TATGACAGGT CGGGCGATGA GACTCTCCTA 1081 CTCCTATGTA ACATCACGTG CTTTCAGAGC CAAGCAGCTG TATGATCTCT ATACGATGCC 1141 TGGCTGCATA TAAATAGCAC ATGGACTCGC A