Construct: ORF ccsbBroadEn_15442

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007611.1_s300c1, BRDN0000394184
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NRG1 (3084)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3084 NRG1 neuregulin 1 NM_013958.3 99.8% 99.5% 113G>A
2 human 3084 NRG1 neuregulin 1 NM_004495.4 87.4% 87.1% 113G>A;633_634ins90
3 human 3084 NRG1 neuregulin 1 NM_001160005.1 85.7% 85% 113G>A;400_401ins102
4 human 3084 NRG1 neuregulin 1 NM_001160002.2 80.3% 79.6% 113G>A;451_452ins51;582_583ins90
5 human 3084 NRG1 neuregulin 1 NM_001160007.2 73.3% 72.6% 113G>A;400_401ins102;531_532ins90
6 human 3084 NRG1 neuregulin 1 NM_001160008.2 56.9% 53.8% (many diffs)
7 human 3084 NRG1 neuregulin 1 NM_001160004.3 52.1% 49.3% (many diffs)
8 human 3084 NRG1 neuregulin 1 NM_013960.5 50.5% 45.5% (many diffs)
9 human 3084 NRG1 neuregulin 1 XM_017013372.2 47.2% 40.4% (many diffs)
10 human 3084 NRG1 neuregulin 1 XM_017013371.2 45.7% 37.1% (many diffs)
11 human 3084 NRG1 neuregulin 1 NM_013962.2 43.9% 39% (many diffs)
12 human 3084 NRG1 neuregulin 1 NM_013957.5 37.5% 35.7% (many diffs)
13 human 3084 NRG1 neuregulin 1 NM_013964.5 36.5% 33.1% (many diffs)
14 human 3084 NRG1 neuregulin 1 NM_013956.5 36.2% 35.8% (many diffs)
15 human 3084 NRG1 neuregulin 1 XM_017013366.2 34.4% 29.7% (many diffs)
16 human 3084 NRG1 neuregulin 1 XM_017013365.2 33.4% 27.4% (many diffs)
17 human 3084 NRG1 neuregulin 1 XM_011544512.2 33.1% 29.8% (many diffs)
18 human 3084 NRG1 neuregulin 1 NM_001159999.3 32.7% 31.3% (many diffs)
19 human 3084 NRG1 neuregulin 1 XM_024447143.1 31.2% 27.8% (many diffs)
20 human 3084 NRG1 neuregulin 1 XM_017013368.2 30.9% 30.3% (many diffs)
21 human 3084 NRG1 neuregulin 1 NM_001159995.3 30% 28.5% (many diffs)
22 human 3084 NRG1 neuregulin 1 XM_017013367.1 28% 24.6% (many diffs)
23 human 3084 NRG1 neuregulin 1 NM_001160001.3 27.4% 25.8% (many diffs)
24 mouse 211323 Nrg1 neuregulin 1 XM_017312638.1 44.4% 42% (many diffs)
25 mouse 211323 Nrg1 neuregulin 1 XM_006509080.3 32.3% 33.1% (many diffs)
26 mouse 211323 Nrg1 neuregulin 1 XM_006509078.3 31.1% 32% (many diffs)
27 mouse 211323 Nrg1 neuregulin 1 XM_006509062.3 23.7% 23.1% (many diffs)
28 mouse 211323 Nrg1 neuregulin 1 XM_006509060.3 23.6% 22.8% (many diffs)
29 mouse 211323 Nrg1 neuregulin 1 XM_006509059.3 23.1% 22.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
789
ORF length:
723
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgtc cgagcgcaaa gaaggcagag gcaaagggaa gggcaagaag aaggagcgag
121gctccggcaa gaagccggag tccgcggcgg gcagccagag cccagccttg cctccccaat
181tgaaagagat gaaaagccag gaatcggctg caggttccaa actagtcctt cggtgtgaaa
241ccagttctga atactcctct ctcagattca agtggttcaa gaatgggaat gaattgaatc
301gaaaaaacaa accacaaaat atcaagatac aaaaaaagcc agggaagtca gaacttcgca
361ttaacaaagc atcactggct gattctggag agtatatgtg caaagtgatc agcaaattag
421gaaatgacag tgcctctgcc aatatcacca tcgtggaatc aaacgagatc atcactggta
481tgccagcctc aactgaagga gcatatgtgt cttcagagtc tcccattaga atatcagtat
541ccacagaagg agcaaatact tcttcatcta catctacatc caccactggg acaagccatc
601ttgtaaaatg tgcggagaag gagaaaactt tctgtgtgaa tggaggggag tgcttcatgg
661tgaaagacct ttcaaacccc tcgagatact tgtgcaagtg cccaaatgag tttactggtg
721atcgctgcca aaactacgta atggccagct tctacagtac gtccactccc tttctgtctc
781tgcctgaatn nccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
841tttgttgcaa cgaac

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