Construct: ORF ccsbBroadEn_16015

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003256.1_s300c1, BRDN0000392009
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM14B (81853)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81853 TMEM14B transmembrane protein 14B NM_030969.5 99.7% 99.1% 124G>T
2 human 51522 TMEM14C transmembrane protein 14C NM_001165258.1 87.7% 72.8% (many diffs)
3 human 51522 TMEM14C transmembrane protein 14C NM_016462.4 87.7% 72.8% (many diffs)
4 human 81853 TMEM14B transmembrane protein 14B NM_001286488.1 71.3% 66.8% (many diffs)
5 human 81853 TMEM14B transmembrane protein 14B NM_001127711.2 70.1% 70.1% 98_99ins102
6 human 81853 TMEM14B transmembrane protein 14B NM_001286484.1 68.3% 65% 1_44del;67_68ins77;91G>T
7 human 81853 TMEM14B transmembrane protein 14B NM_001286489.1 49.1% 45% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
408
ORF length:
342
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgga gaagcccctc ttcccattag tgcctttgca ttggtttggc tttggctaca
121cagcactggt tgtttctggt gggatcgttg gctatgtaaa aacaggcagc gtgccgtccc
181tggctgcatg gctgctcttc ggcagtctag ccggcctggg tgcttaccag ctgtatcagg
241atccaaggaa cgtttggggt ttcctagccg ctacatctgt tacttttgtt ggtgttatgg
301gaatgagatc ctactactat ggaaaattca tgcctgtagg tttaattgca ggtgccagtt
361tgctgatggc cgccaaagtt ggagttcgta tgttgatgac atctgattnn ccaactttct
421tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaac

Download FASTA (ORF) (Full)