| Clone ID |
Target Seq |
Vector |
Matching Transcripts for Gene |
Match Regions[?]The region of the transcript where each match occurs (5UTR, CDS, 3UTR). NOTE: all matches to non-coding transcripts are labeled 3UTR, and the regions here are not necessarily listed in the same order as the matching transcripts. |
SDR Match %[?]Percent match of the "Specificity-Defining Region" of the hairpin target sequence to transcript RNA sequence. The SDR is defined as the initial 19 bases of the (21mer) target sequence.
| 84% | = 16/19 | | 89% | = 17/19 | | 95% | = 18/19 | | 100% | = 19/19 |
|
Intrinsic Score[?]Also called "original score", this assesses the target sequence for predicted cloneability and knockdown performance. |
Adjusted Score[?]Also called "specificity score", this is a function of the intrinsic score and the specificity factor. |
Matches other Human Gene? |
Orig. Target Gene[?]The gene that this construct was originally designed to target |
Addgene[?]Link to this entity's page in the Addgene catalog, if available |
| 1 |
TRCN0000236440 |
ACGCATGATGGTCGGAGTAAA |
pLKO_005 |
NM_007236.5 |
CDS |
100% |
13.200 |
18.480 |
N |
CHP1 |
n/a |
| 2 |
TRCN0000053919 |
CGCATGATGGTCGGAGTAAAT |
pLKO.1 |
NM_007236.5 |
CDS |
100% |
13.200 |
18.480 |
N |
CHP1 |
n/a |
| 3 |
TRCN0000236438 |
GACTCTCAGCCGGGAAGATTT |
pLKO_005 |
NM_007236.5, XM_017021879.2 |
CDS |
100% |
13.200 |
9.240 |
N |
CHP1 |
n/a |
| 4 |
TRCN0000053921 |
GCCATATCTTTCACAGAATTT |
pLKO.1 |
NM_007236.5 |
CDS |
100% |
13.200 |
9.240 |
N |
CHP1 |
n/a |
| 5 |
TRCN0000236437 |
TGTTACGGAAGTGCCTAATAT |
pLKO_005 |
NM_007236.5 |
3UTR |
100% |
15.000 |
9.000 |
N |
CHP1 |
n/a |
| 6 |
TRCN0000236439 |
CGTGGATTCATGCGAACTTTG |
pLKO_005 |
NM_007236.5, XM_017021879.2 |
CDS |
100% |
10.800 |
8.640 |
N |
CHP1 |
n/a |
| 7 |
TRCN0000236441 |
GCATCCGATTTCTTCACTAAA |
pLKO_005 |
NM_007236.5 |
CDS |
100% |
13.200 |
7.920 |
N |
CHP1 |
n/a |
| 8 |
TRCN0000184031 |
CGGGAAGATTTCCAGAGGATT |
pLKO.1 |
NM_007236.5, XM_017021879.2 |
CDS |
100% |
4.950 |
3.465 |
N |
Chp1 |
n/a |
| 9 |
TRCN0000053922 |
CAGTCAAATCACTCGCCTCTA |
pLKO.1 |
NM_007236.5, XM_017021879.2 |
CDS |
100% |
4.050 |
2.835 |
N |
CHP1 |
n/a |
| 10 |
TRCN0000053920 |
CCAGGTAAACTTCCGTGGATT |
pLKO.1 |
NM_007236.5, XM_017021879.2 |
CDS |
100% |
4.950 |
2.475 |
Y |
CHP1 |
n/a |
| 11 |
TRCN0000165534 |
GAGACAGGGTTTCACCATGTT |
pLKO.1 |
NM_007236.5 |
3UTR |
100% |
4.950 |
2.475 |
Y |
|
n/a |
| 12 |
TRCN0000155836 |
CCCAAAGTGCTGGGATTACAA |
pLKO.1 |
NM_007236.5 |
3UTR |
100% |
5.625 |
2.813 |
Y |
KLHL30 |
n/a |
| 13 |
TRCN0000172742 |
GAGACAGAGTCTTGCTCTGTT |
pLKO.1 |
NM_007236.5 |
3UTR |
100% |
0.495 |
0.248 |
Y |
C11orf44 |
n/a |
| 14 |
TRCN0000141025 |
CCCAAAGTGCTGGGATTACTT |
pLKO.1 |
NM_007236.5 |
3UTR |
100% |
5.625 |
2.813 |
Y |
EID2B |
n/a |
| 15 |
TRCN0000148469 |
CTGGGTTCAAGCAATTCTCTT |
pLKO.1 |
NM_007236.5 |
3UTR |
100% |
4.950 |
2.475 |
Y |
C16orf89 |
n/a |