Vector: pLKO.1

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Vector Information:

Vector Name:
pLKO.1
Alias:
pLKO_001, pLKO_TRC001
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
1.9 KB Stuffer
Selection Marker:
PuroR
Visible Reporter:
n/a
Cloning Site:
1.9 KB Stuffer

Sequence Data Files

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GenBank[?] FASTA SnapGene[?] Revision Notes
1 GB FASTA DNA 2017-04-12: Sequence corrected; changes unlikely to have functional consequences
2 GB FASTA n/a (original version)
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Control Clones

Clone ID Target Seq Target Gene ID Target Gene Symbol Taxon Target Region Notes Addgene[?]
1 TRCN0000072189 CCTACGGCGTGCAGTGCTTCA GFP GFP CONTROL CDS
n/a
2 TRCN0000072179 CGACCACATGAAGCAGCACGA GFP GFP CONTROL CDS
n/a
3 TRCN0000072190 GACCACATGAAGCAGCACGAC GFP GFP CONTROL CDS
n/a
4 TRCN0000072200 AGTACAACTACAACAGCCACA GFP GFP CONTROL CDS
n/a
5 TRCN0000207152 AGTTCGTGACCGCCGCCGGGA GFP GFP CONTROL CDS
n/a
6 TRCN0000207114 TCCGCCCTGAGCAAAGACCCC GFP GFP CONTROL CDS
n/a
7 TRCN0000072199 TGACCCTGAAGTTCATCTGCA GFP GFP CONTROL CDS
n/a
8 TRCN0000072188 CCCGACCACATGAAGCAGCAC GFP GFP CONTROL CDS
n/a
9 TRCN0000072178 CAACAGCCACAACGTCTATAT GFP GFP CONTROL CDS
n/a
10 TRCN0000072196 ACGTCTATATCATGGCCGACA GFP GFP CONTROL CDS
n/a
11 TRCN0000072181 ACAACAGCCACAACGTCTATA GFP GFP CONTROL CDS
n/a
12 TRCN0000072180 CTATATCATGGCCGACAAGCA GFP GFP CONTROL CDS
n/a
13 TRCN0000072198 GCGCGATCACATGGTCCTGCT GFP GFP CONTROL CDS
n/a
14 TRCN0000207065 GCGATCACATGGTCCTGCTGG GFP GFP CONTROL CDS
n/a
15 TRCN0000072195 GCGACGTAAACGGCCACAAGT GFP GFP CONTROL CDS
n/a
16 TRCN0000072202 GCCACAACATCGAGGACGGCA GFP GFP CONTROL CDS
n/a
17 TRCN0000207020 GACCACCCTGACCTACGGCGT GFP GFP CONTROL CDS
n/a
18 TRCN0000072187 CTCTCGGCATGGACGAGCTGT GFP GFP CONTROL CDS
n/a
19 TRCN0000072194 CCACATGAAGCAGCACGACTT GFP GFP CONTROL CDS
n/a
20 TRCN0000072186 TGCCCGACAACCACTACCTGA GFP GFP CONTROL CDS
n/a
21 TRCN0000072182 TCTCGGCATGGACGAGCTGTA GFP GFP CONTROL CDS
n/a
22 TRCN0000072185 TACAACAGCCACAACGTCTAT GFP GFP CONTROL CDS
n/a
23 TRCN0000072201 GTCGAGCTGGACGGCGACGTA GFP GFP CONTROL CDS
n/a
24 TRCN0000207257 GCTGTTCACCGGGGTGGTGCC GFP GFP CONTROL CDS
n/a
25 TRCN0000206973 GCACGACTTCTTCAAGTCCGC GFP GFP CONTROL CDS
n/a
26 TRCN0000072192 GAACGGCATCAAGGTGAACTT GFP GFP CONTROL CDS
n/a
27 TRCN0000072193 CGACGTAAACGGCCACAAGTT GFP GFP CONTROL CDS
n/a
28 TRCN0000072183 CGGCATGGACGAGCTGTACAA GFP GFP CONTROL CDS
n/a
29 TRCN0000072184 CGGGATCACTCTCGGCATGGA GFP GFP CONTROL CDS
n/a
30 TRCN0000072197 CTACGGCAAGCTGACCCTGAA GFP GFP CONTROL CDS
n/a
31 TRCN0000072254 ATGTTTACTACACTCGGATAT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
32 TRCN0000072251 GAGTACTTCGAAATGTCCGTT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
33 TRCN0000072258 TGTCCGGTTATGTAAACAATC LUCIFERASE LUCIFERASE CONTROL CDS
n/a
34 TRCN0000072253 ACACTCGGATATTTGATATGT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
35 TRCN0000072260 CAGAATCGTCGTATGCAGTGA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
36 TRCN0000072255 TCTACTGGTCTGCCTAAAGGT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
37 TRCN0000072245 TCACAGAATCGTCGTATGCAG LUCIFERASE LUCIFERASE CONTROL CDS
n/a
38 TRCN0000072262 CACTTACGCTGAGTACTTCGA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
39 TRCN0000072265 GTTGTGTTTGTGGACGAAGTA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
40 TRCN0000072257 TGAGTACTTCGAAATGTCCGT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
41 TRCN0000072261 CACTCGGATATTTGATATGTG LUCIFERASE LUCIFERASE CONTROL CDS
n/a
42 TRCN0000072264 GCTGAGTACTTCGAAATGTCC LUCIFERASE LUCIFERASE CONTROL CDS
n/a
43 TRCN0000072249 GCGGTTGCCAAGAGGTTCCAT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
44 TRCN0000072267 GCGCCATTCTATCCGCTGGAA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
45 TRCN0000072256 ACGCTGAGTACTTCGAAATGT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
46 TRCN0000072266 GAATGTTTACTACACTCGGAT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
47 TRCN0000072247 GAATCGTCGTATGCAGTGAAA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
48 TRCN0000072244 ATCACAGAATCGTCGTATGCA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
49 TRCN0000072243 CTTCGAAATGTCCGTTCGGTT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
50 TRCN0000072248 AGTCAAGTAACAACCGCGAAA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
51 TRCN0000072250 AGAATCGTCGTATGCAGTGAA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
52 TRCN0000072252 ACTTACGCTGAGTACTTCGAA LUCIFERASE LUCIFERASE CONTROL CDS
n/a
53 TRCN0000072259 CGCTGAGTACTTCGAAATGTC LUCIFERASE LUCIFERASE CONTROL CDS
n/a
54 TRCN0000072246 CAAATCACAGAATCGTCGTAT LUCIFERASE LUCIFERASE CONTROL CDS
n/a
55 TRCN0000072263 AGTACTTCGAAATGTCCGTTC LUCIFERASE LUCIFERASE CONTROL CDS
n/a
56 TRCN0000072212 CCGTAATGCAGAAGAAGACCA RFP RFP CONTROL CDS
n/a
57 TRCN0000072203 CGCGTGATGAACTTCGAGGAC RFP RFP CONTROL CDS
n/a
58 TRCN0000072211 CTACACCATCGTGGAACAGTA RFP RFP CONTROL CDS
n/a
59 TRCN0000072205 GCTCCGTGAACGGCCACGAGT RFP RFP CONTROL CDS
n/a
60 TRCN0000072215 GTAATGCAGAAGAAGACCATG RFP RFP CONTROL CDS
n/a
61 TRCN0000072206 GTGGGAGCGCGTGATGAACTT RFP RFP CONTROL CDS
n/a
62 TRCN0000072204 TCAGTTCCAGTACGGCTCCAA RFP RFP CONTROL CDS
n/a
63 TRCN0000072221 TGCAGAAGAAGACCATGGGCT RFP RFP CONTROL CDS
n/a
64 TRCN0000072217 CAACGAGGACTACACCATCGT RFP RFP CONTROL CDS
n/a
65 TRCN0000072210 CGTAATGCAGAAGAAGACCAT RFP RFP CONTROL CDS
n/a
66 TRCN0000072219 CTACAAGACCGACATCAAGCT RFP RFP CONTROL CDS
n/a
67 TRCN0000072209 CTCAGTTCCAGTACGGCTCCA RFP RFP CONTROL CDS
n/a
68 TRCN0000072218 GAACGGCCACGAGTTCGAGAT RFP RFP CONTROL CDS
n/a
69 TRCN0000072213 ACTACACCATCGTGGAACAGT RFP RFP CONTROL CDS
n/a
70 TRCN0000072216 CCACTACGACGCCGAGGTCAA RFP RFP CONTROL CDS
n/a
71 TRCN0000072208 GCTTCAAGTGGGAGCGCGTGA RFP RFP CONTROL CDS
n/a
72 TRCN0000072233 CGACCACGCAAATCAGCGATT lacZ lacZ CONTROL CDS
n/a
73 TRCN0000072224 CGCGATCGTAATCACCCGAGT lacZ lacZ CONTROL CDS
n/a
74 TRCN0000072240 TCGTATTACAACGTCGTGACT lacZ lacZ CONTROL CDS
n/a
75 TRCN0000072226 CGATCGTAATCACCCGAGTGT lacZ lacZ CONTROL CDS
n/a
76 TRCN0000072228 ACTCTGGCTAACGGTACGCGT lacZ lacZ CONTROL CDS
n/a
77 TRCN0000072229 GCGATCGTAATCACCCGAGTG lacZ lacZ CONTROL CDS
n/a
78 TRCN0000072239 GCCGTCGTATTACAACGTCGT lacZ lacZ CONTROL CDS
n/a
79 TRCN0000072234 CGGATTCTCTGGCCGTCGTAT lacZ lacZ CONTROL CDS
n/a
80 TRCN0000072231 CGCTAAATACTGGCAGGCGTT lacZ lacZ CONTROL CDS
n/a
81 TRCN0000072235 CCGTCATAGCGATAACGAGTT lacZ lacZ CONTROL CDS
n/a
82 TRCN0000072230 CCCGTCAGTATCGGCGGAATT lacZ lacZ CONTROL CDS
n/a
83 TRCN0000072237 CGCGCCTTTCGGCGGTGAAAT lacZ lacZ CONTROL CDS
n/a
84 TRCN0000072232 CGTCGTATTACAACGTCGTGA lacZ lacZ CONTROL CDS
n/a
85 TRCN0000072225 CTCTGGCTAACGGTACGCGTA lacZ lacZ CONTROL CDS
n/a
86 TRCN0000072227 GCGCTAATCACGACGCGCTGT lacZ lacZ CONTROL CDS
n/a
87 TRCN0000072241 GCGTTGGCAATTTAACCGCCA lacZ lacZ CONTROL CDS
n/a
88 TRCN0000072242 GTCGGCTTACGGCGGTGATTT lacZ lacZ CONTROL CDS
n/a
89 TRCN0000072238 GTTCCGTCATAGCGATAACGA lacZ lacZ CONTROL CDS
n/a
90 TRCN0000072223 TGTTCGCATTATCCGAACCAT lacZ lacZ CONTROL CDS
n/a
91 TRCN0000072236 CCAACGTGACCTATCCCATTA lacZ lacZ CONTROL CDS
n/a
92 TRCN0000222901 ACCGACAAGATCATCCGCAGC tGFP tGFP CONTROL CDS
n/a
93 TRCN0000222866 GGACAGCCACATGCACTTCAA tGFP tGFP CONTROL CDS
n/a
94 TRCN0000222885 GCCACGTGATGGGCTACGGCT tGFP tGFP CONTROL CDS
n/a
95 TRCN0000222803 ACCTGCTGAGCCACGTGATGG tGFP tGFP CONTROL CDS
n/a
96 TRCN0000222819 CGGCTACTACAGCTCCGTGGT tGFP tGFP CONTROL CDS
n/a
97 TRCN0000223035 GCGTGATCTTCACCGACAAGA tGFP tGFP CONTROL CDS
n/a
98 TRCN0000222796 TCCGTGGTGGACAGCCACATG tGFP tGFP CONTROL CDS
n/a
99 TRCN0000204879 (no hairpin) n/a n/a n/a
n/a
100 TRCN0000208001 (no hairpin) n/a n/a n/a
n/a
101 TRCN0000241922 (no hairpin) n/a n/a n/a
n/a
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