Gene Search Results 2: Clones Refine Your Search
sgRNA constructs matching to requested genes (CRISPRko, NGG PAM)
Matching is performed by first choosing a representative transcript for each gene, and then performing sequence matching for the full 20mer preceding an NGG PAM sequence. Since this method selects only a single transcript for each gene, it may miss some constructs that match other transcripts for the gene.
shRNA constructs with 100% match to requested genes
Matching is performed using the Specificity-Defining Region (SDR)[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity. of the shRNAs. This list includes matches to any current transcript from a requested gene, regardless of what transcript the shRNAs were originally designed to target. For example, some shRNAs in this list may have been originally designed to target: (i) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (ii) a transcript of a different gene from the same or different taxon.