Oligo Design for Target Sequence 'CGGAAGAAGCCAGTTAAGAGA'
Forward Oligo:
- 5' flank sequence:
- CCGG
- Loop sequence:
- CTCGAG
- 3' flank sequence:
- TTTTTG
- Target sequence:
- CGGAAGAAGCCAGTTAAGAGA
- Reverse complement target sequence:
- TCTCTTAACTGGCTTCTTCCG
- Full sequence:
- 5'-CCGGCGGAAGAAGCCAGTTAAGAGACTCGAGTCTCTTAACTGGCTTCTTCCGTTTTTG-3'
Reverse Oligo:
- 5' flank sequence:
- AATTCAAAAA
- Loop sequence:
- CTCGAG
- 3' flank sequence:
- (none)
- Full sequence:
- 5'-AATTCAAAAACGGAAGAAGCCAGTTAAGAGACTCGAGTCTCTTAACTGGCTTCTTCCG-3'