Protocols
Informational Guides
| Guide | Date | Link | |
|---|---|---|---|
| 1 | Selecting a Genome-wide Library | 20 Jan 2023 | Google doc |
| 2 | Ordering Lentiviral Libraries | 27 Jan 2023 | Google doc |
Video Protocols
| Video Topic | Date | Link | |
|---|---|---|---|
| 1 | Creation of a Pooled Library | 27 Nov 2017 | YouTube video |
| 2 | Large Scale Virus Infection | 27 Nov 2017 | YouTube video |
| 3 | Viral Titration | 27 Nov 2017 | YouTube video |
| 4 | Pooling Large Scale Infections | 27 Nov 2017 | YouTube video |
Cloning and Plasmid DNA (pDNA) Production
| Protocol Name | Date | Download | |
|---|---|---|---|
| 1 | shRNA/sgRNA Cloning Protocol | 30 Nov 2019 | |
| 2 | shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation | 30 Sep 2015 | |
| 3 | pDNA Library Amplification | 22 Oct 2024 |
Virus Production and Titering
| Protocol Name | Date | Download | |
|---|---|---|---|
| 1 | shRNA/sgRNA/ORF High Throughput Viral Production (96 well) | 9 Jan 2018 | |
| 2 | shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) | 3 Jun 2015 | |
| 3 | Lentiviral Production in Flasks | 27 Nov 2023 | |
| 4 | Viral Titering Protocol (alamarBlue) | 21 Sep 2016 | |
| 5 | Puromycin, Blasticidin and Hygromycin Titration Protocol | 23 Sep 2020 | |
| 6 | Lentivirus Concentration Protocol | 11 Feb 2019 |
Cas9 Assessment
| Protocol Name | Date | Download | |
|---|---|---|---|
| 1 | Cas9 detection using flow cytometry | 01 Feb 2019 | |
| 2 | Cas9/Cas12a Activity Assay | 23 Sep 2020 | |
| 3 | dCas9 Activity Assay for CRISPRa Screening | 23 Sep 2020 | |
| 4 | dCas9 Activity Assay for CRISPRi Screening | 23 Sep 2020 | |
| 5 | Out-of-frame eGFP CRISPR Gene Editing Reporter Assay | 30 Sep 2015 |
Viral Infection
| Protocol Name | Date | Download | |
|---|---|---|---|
| 1 | Optimization of Lentiviral Spinfection | 23 Sep 2020 | |
| 2 | No-Spin Infection Protocol | 11 Feb 2019 | zip |
gDNA Isolation, PCR and Next-Generation Sequence Data Analysis
| Protocol Name | Date | Download | |
|---|---|---|---|
| 1 | gDNA Isolation Protocol with NucleoSpin Blood Kits and PCR pre-check | 09 Apr 2021 | |
| 2 | sgRNA/shRNA/ORF PCR for Illumina Sequencing | 22 Jan 2024 | zip |
| 3 | Pooled Screening Deconvolution Using PoolQ (old version: PoolQ 2.x) | 20 Dec 2019 | |
| 4 | Pooled Screening Deconvolution Using PoolQ (current version: PoolQ 3.x) | 20 Dec 2019 | |
| 5 | Pooled Screen Analysis Guide | 22 Oct 2020 |