Protocols

Video Protocols

Video Topic Date Link
1 Creation of a Pooled Library 27 Nov 2017 YouTube video
2 Large Scale Virus Infection 27 Nov 2017 YouTube video
3 Viral Titration 27 Nov 2017 YouTube video
4 Pooling Large Scale Infections 27 Nov 2017 YouTube video

Cloning and Plasmid DNA (pDNA) Production

Protocol Name Date Download
1 shRNA/sgRNA Cloning Protocol 30 Nov 2019 pdf
2 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015 pdf
3 pDNA Library Amplification 23 Sep 2020 pdf

Virus Production and Titering

Protocol Name Date Download
1 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 9 Jan 2018 pdf
2 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
3 Cas9/shRNA/sgRNA/ORF Large Scale Lentiviral Production (T-175 flasks) 11 Feb 2019 pdf
4 Viral Titering Protocol (alamarBlue) 21 Sep 2016 pdf
5 Puromycin, Blasticidin and Hygromycin Titration Protocol 23 Sep 2020 pdf
6 Lentivirus Concentration Protocol 11 Feb 2019 pdf

Cas9 Assessment

Protocol Name Date Download
1 Cas9 detection using flow cytometry 01 Feb 2019 pdf
2 Cas9/Cas12a Activity Assay 23 Sep 2020 pdf
3 dCas9 Activity Assay for CRISPRa Screening 23 Sep 2020 pdf
4 dCas9 Activity Assay for CRISPRi Screening 23 Sep 2020 pdf
5 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015 pdf

Viral Infection

Protocol Name Date Download
1 Optimization of Lentiviral Spinfection 23 Sep 2020 pdf
2 No-Spin Infection Protocol 11 Feb 2019 zip

gDNA Isolation, PCR and Next-Generation Sequence Data Analysis

Protocol Name Date Download
1 gDNA Isolation Protocol with NucleoSpin Blood Kits and PCR pre-check 23 Sep 2020 pdf
2 sgRNA/shRNA/ORF PCR for Illumina Sequencing 19 Jun 2020 zip
3 Pooled Screening Deconvolution Using PoolQ (old version: PoolQ 2.x) 20 Dec 2019 pdf
4 Pooled Screening Deconvolution Using PoolQ (current version: PoolQ 3.x) 20 Dec 2019 pdf
5 Introduction to Pooled Screen Analysis 20 Dec 2019 pdf