Protocols

Video Protocols

Video Topic Date Link
1 BroadE: GPP Workshop Playlist 27 Nov 2017 YouTube playlist
2 Creation of a Pooled Library 27 Nov 2017 YouTube video
3 Large Scale Virus Infection 27 Nov 2017 YouTube video
4 Viral Titration 27 Nov 2017 YouTube video
5 Pooling Large Scale Infections 27 Nov 2017 YouTube video

Cloning and Plasmid DNA (pDNA) Production

Protocol Name Date Download
1 shRNA/sgRNA Cloning Protocol 30 Sep 2015 pdf
2 shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation 30 Sep 2015 pdf
3 pDNA Library Amplification 25 Jan 2018 pdf

Virus Production and Titering

Protocol Name Date Download
1 shRNA/sgRNA/ORF High Throughput Viral Production (96 well) 9 Jan 2018 pdf
2 shRNA/sgRNA/ORF Low Throughput Viral Production (10cm dish/6 well) 3 Jun 2015 pdf
3 shRNA/sgRNA/ORF Large Scale Lentiviral Production (T-175 flasks) 9 Jan 2018 pdf
4 Viral Titering Protocol (alamarBlue) 21 Sep 2016 pdf
5 Puromycin and Blasticidin Titration Protocol 27 May 2018 pdf
6 Virus Concentration Protocol 14 Feb 2018 pdf

Cas9 Activity Assessment

Protocol Name Date Download
1 Cas9 Activity Assay 17 Nov 2017 pdf
2 dCas9 Activity Assay for CRISPRa Screening 25 Sep 2018 pdf
3 Out-of-frame eGFP CRISPR Gene Editing Reporter Assay 30 Sep 2015 pdf

Viral Infection

Protocol Name Date Download
1 Optimization of Lentiviral Spinfection 23 Jul 2018 pdf
2 No-Spin Infection Protocol 21 Jun 2016 zip

gDNA Isolation, PCR and Next-Generation Sequence Data Analysis

Protocol Name Date Download
1 gDNA Miniprep Isolation Protocol 1 Sep 2016 pdf
2 gDNA Maxiprep Isolation Protocol(NucleoSpin Blood XL-Maxi Kit) 15 May 2018 pdf
3 gDNA Sample Submission Protocol 31 Jul 2018 zip
4 sgRNA/shRNA/ORF PCR for Illumina Sequencing 3 Apr 2018 zip
5 Pooled Screening Deconvolution Using PoolQ 2 Mar 2016 pdf
6 Introduction to Pooled Screen Analysis 17 Apr 2017 pdf