Summary Tables

Informational Guides Project Planning Assay Development Screening Post-Screen Analysis Screen Follow-Up

GPP Collaboration Guide
Selecting a Genome-Wide Library
Druggable CRISPR Libraries
Ordering Pooled Libraries
Adding Internal Barcodes (iBars) to a Custom Library
Ordering 96-well Arrayed Constructs - sgRNAs
Ordering Individual or 96-well Arrayed Constructs - shRNAs
Ordering Individual or 96-well Arrayed Constructs - ORFs
Ordering 96-well Custom Constructs
Designing a Pooled Library with CRISPick
Designing a Multi-Guide Cas12a Library
Designing a Dual-Guide Cas9 Library
Designing a Base Editor Library with Beagle
Screening with Deep Mutational Scanning
Vectors for Assay Development
Adding a New pF Fragment to Fragmid
VPX Viral-Like Particles
Coverage Considerations In Pooled Genetic Screens
Barcoding Libraries: Cell Counter and CloneSifter
Screen Deconvolution with PoolQ
How to Pick a .chip File from the GPP
Pooled Screen Analysis with Apron
Base Editing Analysis Guide
Protocols Project Planning Assay Development Screening Post-Screen Analysis Screen Follow-Up

Vector Transformation
Optimization of Lentiviral Spinfection
Optimization of Lentiviral No-Spin Infection
Puromycin, Blasticidin and Hygromycin Titration
Cas Activity Assay for CRISPRko Screening
Cas Activity Assay for CRISPRko All-in-One Screening
dCas Activity Assay for CRISPRa or CRISPRi Screening
Base Editor Activity Assay for CRISPRbe Screening
Cas Detection Using Flow Cytometry
Pooled Screen Spinfection Viral Titration
Pooled Screen No-Spin Viral Titration
Cloning of sgRNA or shRNA Constructs
shRNA/sgRNA/ORF Glycerol and Plasmid DNA Preparation
pDNA Library Amplification
Lentiviral Production in 96-well plates
Lentiviral Production in 6-well Plates or 10 cm Dishes
Lentiviral Production in Flasks
Lentiviral Concentration
Functional Viral Titering Assay
Production of VPX Viral-Like particles
Genomic DNA Isolation and PCR Pre-Check
PCR of sgRNAs/shRNAs/ORFs from gDNA for Illumina Sequencing
Order Forms Project Planning Assay Development Screening Post-Screen Analysis Screen Follow-Up

Individual Vectors
Cell Lines
sgRNAs
shRNAs/ORFs

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