Transcript: Human NM_001144060.2

Homo sapiens NHS like 1 (NHSL1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
NHSL1 (57224)
Length:
7063
CDS:
205..5025

Additional Resources:

NCBI RefSeq record:
NM_001144060.2
NBCI Gene record:
NHSL1 (57224)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001144060.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000337081 CAAGAGTGAATCTAGTTATTC pLKO_005 1809 CDS 100% 13.200 18.480 N NHSL1 n/a
2 TRCN0000337004 CTCTGGTCTAGGCGGTAATTG pLKO_005 5422 3UTR 100% 13.200 18.480 N NHSL1 n/a
3 TRCN0000337006 CCCAAGCTATGCCGTATAATT pLKO_005 1742 CDS 100% 15.000 10.500 N NHSL1 n/a
4 TRCN0000337014 GGCTGACGTGGTGCCTATTAA pLKO_005 708 CDS 100% 15.000 10.500 N NHSL1 n/a
5 TRCN0000337003 AGTGACTTCTCCGACCTTAAT pLKO_005 610 CDS 100% 13.200 9.240 N NHSL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001144060.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.