Transcript: Mouse NM_001305042.1

Mus musculus sperm flagellar 2 (Spef2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Spef2 (320277)
Length:
5565
CDS:
100..5496

Additional Resources:

NCBI RefSeq record:
NM_001305042.1
NBCI Gene record:
Spef2 (320277)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001305042.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000250543 AGAGCCCAACAGCGTTATAAG pLKO_005 1348 CDS 100% 13.200 18.480 N Spef2 n/a
2 TRCN0000250545 CTTGCGGTCAAGGGTTGTATA pLKO_005 1771 CDS 100% 13.200 18.480 N Spef2 n/a
3 TRCN0000250546 TCATCGTTGGCTCGAACTAAT pLKO_005 2437 CDS 100% 13.200 10.560 N Spef2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001305042.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.