Construct: shRNA TRCN0000231711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
lacZ_3003s21c1
DNA Barcode:
CCCGTCAGTATCGGCGGAATT

Original Target:

Original Target:

Taxon:
Control
Gene:
lacZ (lacZ)
Gene Description:
lacZ
Transcript:
Broad lacZ.1 (CURRENT)
Match location:
Position 3003 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this hairpin:

Taxon Gene Symbol Description Transcript SDR Match %[?] Region Start Pos.[?] Intrinsic Score[?] Adjusted Score[?]
1 CONTROL lacZ lacZ lacZ lacZ.1 100% CDS 3003 0.000 N/A
2 CONTROL LacZ LacZ Hahn Lab LacZ LacZ.1 100% CDS 2988 0.000 N/A
Download CSV

Sequence Information

Target Sequence:
CCCGTCAGTATCGGCGGAATT
Hairpin Sequence:
5'-CCGG-CCCGTCAGTATCGGCGGAATT-CTCGAG-AATTCCGCCGATACTGACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTCAGTATCGGCGGAATTCTCGAGAATTCCGCCGATACTGACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTCAGTATCGGCGGAATTCTCGAGAATTCCGCCGATACTGACGGG-3'

Other clones with same target sequence:

  1. TRCN0000072230