Construct: shRNA TRCN0000231725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
rfp_620s21c1
DNA Barcode:
ACTACACCATCGTGGAACAGT

Original Target:

Taxon:
Control
Gene:
RFP (RFP)
Gene Description:
RFP
Transcript:
Broad rfp.1 (CURRENT)
Match location:
Position 620 (CDS)

Vector Information:

Vector Backbone:
pLKO_TRC005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTACACCATCGTGGAACAGT
Hairpin Sequence:
5'-CCGG-ACTACACCATCGTGGAACAGT-CTCGAG-ACTGTTCCACGATGGTGTAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTACACCATCGTGGAACAGTCTCGAGACTGTTCCACGATGGTGTAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTACACCATCGTGGAACAGTCTCGAGACTGTTCCACGATGGTGTAGT-3'

Other clones with same target sequence:

  1. TRCN0000072213